腹腔镜CO_2气腹对人宫颈癌CaSki细胞中凋亡抑制因子表达的影响  

Effect of carbon dioxide pneumoperitoneum on the expressions of survivin in human cervical carcinoma cell line

作　　者：干晓琴[1] 赵纯全[2] 姚珍薇[2] 

机构地区：[1]四川省成都市妇产科医院妇产科,成都610015 [2]重庆医科大学附属第一医院妇产科

出　　处：《陕西医学杂志》2009年第6期660-662,共3页Shaanxi Medical Journal

摘　　要：目的:研究CO2气腹对人宫颈癌CaSki细胞中survivin mRNA和蛋白表达的影响,探讨其促肿瘤生长的分子机制。方法:建立腹腔镜CO2气腹体外模型,以不同压力(0mmHg、7mmHg、14mmHg)的CO2气腹对人宫颈癌CaSki细胞处理不同时间(1h、2h、3h、4h),设未处理组为对照。采用RT-PCR法和免疫细胞化学法分别检测处理后各组细胞survivin mRNA和蛋白表达情况。结果:CO2气腹在一定的压力和作用时间范围上调CaSki细胞中survivin mRNA和蛋白的表达。结论:CO2气腹促肿瘤生长的效应可能是通过抑制肿瘤细胞的凋亡而实现的。Objective：To study the effect of CO2 pneumoperitoneum used in laparoscopy on the expressions of survivin mRNA and protein in human cervical carcinoma cell line （CaSki） . Methods：The CO2 pneumoperitoneum model was established in vitro. After CaSki cells were exposed to carbon dioxide pneumoperitoneum at 0mmHg, 7mmH scope or 14mmHg for 1h, 2h , 3h or 4h with the untreated group as control, the expressions of survivin mRNA and protein were examined by RT-PCR and immunocytochemistry. Results： Within certain pressure and exposure time, carbon dioxide pneumoperitoneums significantly upregulated the expressions of survivin mRNA and protein in CaSki cells. Conclusion：The promoting effect of carbon dioxide pneumoperitoneum on malignant cells growth is probably through inhibiting the apoptosis of tumor cells.

关 键 词：宫颈肿瘤 二氧化碳 气腹 @survivin 

分 类 号：R614[医药卫生—麻醉学] R737.33[医药卫生—外科学]