鲤鱼源粪肠球菌增菌培养基的优化  

Optimization of enrichment medium for Enterococcus Faecalis from carp

作　　者：单晓枫[1] 张洪波[1] 钱爱东[1] 韩云 

机构地区：[1]吉林农业大学动物科学技术学院,130118 [2]吉林省白城市通榆县瞻榆镇畜禽防疫服务中心

出　　处：《中国饲料》2009年第10期20-23,共4页China Feed

基　　金：国家自然科学基金资助项目(30471286)

摘　　要：利用SAS软件研究鲤鱼源粪肠球菌(Enterococcus faeclis)YL-1增菌培养基,首先,通过Plackett-Burman试验设计在培养基的各个因子中筛选出对YL-1生长有利的重要因子,然后在Box-Behnken试验设计下确定最大响应值,并通过优化的结果验证。结果表明预测值和实际值具有良好的拟合性,优化模型可靠,确定YL-1最佳培养基为:蛋白胨1.365%、牛肉膏1.25%、酵母浸粉0.625%、麦芽糖2.5%、吐温-800.1%、KH2PO40.2%、柠檬酸三铵0.16%、MgSO40.25%、初始pH值6.4。培养优化后YL-1的菌体浓度达1.71×109CFU/mL。The optimization medium for Enterococcus faeclis YL-1 was studied with SAS system. Firstly,the important factors favorable to the growth of YL-1 were chosen by Plackett-Burman design;The maximum response value was identified by Box-Behnken design.It showed the estimated value had good fitting with the real value ,so the model was reliable.The optimization of cultured medium of YL-1 was as follows： 1.365 % peptone, 1.25 % extractum carnis,0.625 % yeast powder,2.5 % mahose,0.1% Tween-80,0.2 KH2PO4,0.16 % ammonium citrate,0.25 % MgSO4,incipient pH 6.4. The content of bacterium in the optimized culture medium reached 1.71×10^9 CFU/mL.

关 键 词：粪肠球菌 Plackett—Burman设计法 Box—Behnken设计法 培养基优化 

分 类 号：S816.7[农业科学—饲料科学]