气相色谱法对血浆及尿中砷的测定  

Determination of the Arsenic in Plasma and Urine by Gas Chromatography

作　　者：秦燕[1] 刘海卫[1] 

机构地区：[1]上海第二医科大学测试中心,200025

出　　处：《上海第二医科大学学报》1998年第3期216-218,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：介绍一种用气相色谱法测定血浆及尿中砷的方法,血浆或尿液处理后,砷(Ⅲ在1mol/L HCl 溶液中与二巯基丙醇(BAL反应后,形成五元环状化合物,经甲苯萃取后测定。得到成年正常人血浆和尿中砷的含量分别为0.334±0.016μmol/L 和0.445±0.198μmol/L,线性范围0.5～150pmol,r=0.9993,检出限为0.00015pmol/ml,回收率在0.95～1.04之间,变异系数0.016。本方法特异性好,操作简便迅速,血硒和尿硒均不干扰,灵敏度高,适合临床分析研究和应用。A method for determination of the arsenic in plasma and urine by gas chr- omatography was developed.In 1 mol/L HCL solution of As(Ⅲ)was reacted with BAL to form a five-element ring compound.The compound was directly determined by GC after extracted by toluene.The result shows that normal values of the arsenic in plasma and urine are 0.334±0.016 and 0.445±0.198 umol/L.The linear range is from 0.5 μmol to 150 pmol.The minimum detection limit of the arsenic is 0.00015 pmol.The analytical recovery is from 0.95 to 1.04,and CV is 0.016.The serum in the samples does not disturb the determination.It was stable、simple and suitable for clinical research and application.

关 键 词：血液 尿液 砷 测定 气相色谱法 

分 类 号：R446.1[医药卫生—诊断学]