肾上腺外组织合成醛固酮  

Aldosterone biosynthesis in extraadrenal tissues

作　　者：吴平生[1] 梁欣伟[1] 戴云 刘宏[1] 臧燕[1] 郭志刚[1] 张榕华 赖文岩[1] 张远慧[1] 刘伊丽[1] 

机构地区：[1]第一军医大学附属南方医院心内科

出　　处：《中华心血管病杂志》1998年第2期139-141,共3页Chinese Journal of Cardiology

基　　金：广东省自然科学基金

摘　　要：目的为证明不仅血管、大脑，而且其它肾上腺外组织合成醛固酮假设的可能性。方法进行了两项实验研究：（一）确定肾脏能否合成醛固酮。用离体肾灌注模型，观察血管紧张素转换酶抑制剂（ＡＣＥＩ）及肾上腺切除对离体肾灌注醛固酮产量的作用，在３０分钟平衡之后，收集１２０ｍｌ灌注液行反向高效液相分离及放免检测。研究表明肾脏离体灌注可以释放醛固酮、肾上腺切除不会改变肾灌注液中的醛固酮产量、而用ＡＣＥＩ培哚普利则可减少其产量；用ＲＴＰＣＲ及ＳｏｕｔｈｅｒｎＢｌｏｔ法证明了肾组织及培养的肾小管上皮细胞能表达醛固酮合成酶基因ＣＹＰ１１Ｂ２ｍＲＮＡ。（二）确定肝脏和肺脏能否合成醛固酮。结果用ＲＴＰＣＲ及ＳｏｕｔｈｅｒｎＢｌｏｔ法证明了肝脏和肺脏能表达ＣＹＰ１１Ｂ２，原位杂交表明ＣＹＰ１１Ｂ２ｍＲＮＡ定位在肝脏的伊藤细胞及肺脏的Ⅱ型肺泡上皮细胞的胞浆内。结论研究证明了肾脏、肝脏及肺脏能够合成醛固酮。Objective To determine the hypothetical possibility that extra adrenal tissues could also synthesize aldosterone besides the vascular tissue and brain. Methods Aldosterone released into the perfusion circuit from the exvivo perfused rat kidney model, and the effects of ACEI and adrenalectomized rats prior to the perfusion experiment on aldosterone production from the kidney model were studied. After 30 min equilibration 120 ml of the perfusate was collected and subjected to reverse phase HPLC. Production of aldosterone in the kidney perfusate was determined by RIA. The expression of aldosterone synthase gene CYP11B2 mRNA was measured by RT PCR, Southern blot and in situ hybridization. Results Production of aldosterone in the kidney perfusate showed no change in adrenalectomized rats, but it was decreased in the kidney perfusate from rats pretreated with ACEI (perindopril). The expression of CYP11 B2 mRNA was demonstraled in both kidney tissue and cultured renal tubular epithelial cell. CYP 11B2 mRNA expression was identified in the liver and lung of rats. In situ hybridization showed that CYP11B2 mRNA was localized in the endoplasm of liver fat storing cell (Ito cells) and type Ⅱ alveolar cells of the lung .Conclusion These studies indicate that kidney, liver and lung are able to produce aldosterone.

关 键 词：醛固酮 原位杂交 合成 肾上腺外组织 

分 类 号：R586.02[医药卫生—内分泌]