甲基强的松龙对海水吸入型急性肺损伤大鼠的保护作用  

Protective effects of methylprednisolone on acute lung injury induced by seawater in rats

作　　者：邓朝霞[1] 钱桂生[1] 杨昱[1] 周长喜[1] 王关嵩[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,重庆400038

出　　处：《中国急救医学》2009年第6期525-528,共4页Chinese Journal of Critical Care Medicine

基　　金：军队“十一五”攻关课题（No.06G083）;国家自然科学基金（No.30770928）

摘　　要：目的观察甲基强的松龙对海水吸入型急性肺损伤（SW-ALI）大鼠的保护作用。方法将60只Wistar大鼠分为三组：对照组、海水组、甲基强的松龙（MP）组，海水组吸入海水（4mL／kg）建立Au模型，MP组于模型建立后15min尾静脉注射MP30mS／kg。分别于模型建立后30、60、120、240、480min进行动脉血气分析，最后检测肺微血管通透性（PMVP）、肺湿／干质量比（W／D）、肺组织髓过氧化物酶（MPO）、丙二醛（MDA）、Na^＋-K^＋-ATP酶（NKA）活性和血浆TNF-α，并观察病理学变化。结果在吸人海水后，PaO2迅速下降，PMVP、W／D、MPO、MDA和血TNF-α显著增高，NKA活性显著降低；MP组的PaO2和NKA活性显著高于海水组（P〈0．05），而PMVP、W／D、MPO、MDA和血TNF-α则显著低于海水组（P〈0．05）。结论早期使用甲基强的松龙可减轻海水吸人大鼠肺部炎症反应和肺水肿，提高氧合。Objective To investigate the protective effects of methylprednisolone on seawater inhalation induced acute lung injury （ SW - ALl） in rat. Methods Sixty Wistar rats were randomly divided into 3 groups： control group, seawater group which inhaled seawater （4 mL/kg ） to induce SW- ALI and methylprednisolone （MP） group which were injected methylprednisolone 30 mg/kg after 15 min of SW - ALI model established. Then PaO2, PaCO2 and pH were monitered at 30 min, 60 min, 120 min, 240 min and 480 min point after SW - ALl model established. Pulmonary microvascular permeability （PMVP） , lung wet/dry weight ratio （ W/D ）, myeloperoxidase （ MPO ） activity, malondialdehyde （ MDA ）, Na^＋ - K^＋- ATPase activity （ NKA ） of lung homogenate and level of TNF-α in plasma were examined by biochemical methods to assess the injury of the lung. Results PaO2 decreased rapidly after seawater inhalation. At 30 min point, PaO2 decreased down to （ 40. 32±4. 96 ） mm Hg which was the lowest parameter after ALI model established. Then PaO2 increased gradually, but the parametem were significant lower than those of control group till to 480 min. PaO2 increased to （ 49. 33 ±6. 07 ） mm Hg after 120 min of methylprednisolone administration, which were significant higher than those of seawater group （P 〈 0. 01 ）. NKA decreased from （6. 85 ±0. 34） μmol/（ mg·h） to （3.12 ±0. 22）μmol/（ mg·h） after seawater inhalation and increased to （ 4. 24 ± 0. 27 ） μmol/（ mg · h ） after methylprednisolone administration which was higher significant than that of seawater group （ P 〈0. 01）. Whereas PMVP, W/D, MPO, MDA and TNF -α increased to （ 85.59 ± 12. 63 ）μg/g, （ 6. 58 ± 0. 29 ） U/g, （ 5.39 ± 0. 37 ） U/g, （ 5.83 ~ 0.48） nmol/mg , （94.75 ± 12.37） pg/mL respectively after seawater inhalation, and decreased to （64.32±8.84））μg/g, （4.82±0.26）U/g, （3.42±0.31）U/g, （4.56±0.51）nmol/mg, （28.38± 4. 69）pg/mL respectively after methy

关 键 词：海水 急性肺损伤 甲基强的松龙 

分 类 号：R563[医药卫生—呼吸系统]