血红素氧合酶-1在感染性休克大鼠肾功能损伤中的作用  

Role of heme oxygenase-1 in renal function injury during septic shock in rats

作　　者：余剑波[1] 姚尚龙[2] 宫丽荣[1] 陈慧荣[1] 王曼[1] 周芬[1] 高宝来[1] 

机构地区：[1]天津市南开医院麻醉科,300100 [2]华中科技大学同济医学院附属协和医院麻醉科

出　　处：《中华麻醉学杂志》2009年第5期463-466,共4页Chinese Journal of Anesthesiology

摘　　要：目的探讨血红素氧合酶-1（HO-1）在感染性休克大鼠肾功能损伤中的作用。方法健康清洁级SD大鼠80只，月龄3～4月，体重260～330g，雌雄不拘，随机分为4组（n=20）：对照组（C组）、感染性休克组（S组）、感染性休克＋ZnPP-Ⅸ组（SZ组）和ZnPP-Ⅸ组（Z组）。C组静脉注射生理盐水0．5ml，2h后腹腔注射50mmol／L碳酸氢钠溶液1ml；S组静脉注射LPS10mg／kg，2h后腹腔注射50mmol／L碳酸氢钠溶液1ml；SZ组静脉注射LPS10mg／kg，2h后腹腔注射ZnPP-Ⅸ10μmol／kg；Z组静脉注射生理盐水0．5ml，2h后腹腔注射ZnPP-Ⅸ10μmol／kg。于腹腔注射碳酸氢钠溶液或ZnPP-Ⅸ后4h时，检测碳氧血红蛋白浓度（COHb）、血清尿素氮（BUN）、肌酐（Cr）的浓度和尿％．微球蛋白（α1-M）浓度、N-乙酰-β-D-氨基葡萄糖酐酶（NAG）活性、视黄醇结合蛋白（RBP）浓度、弘谷氨酰转肽酶（γ-GT）活性、肾组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性、HO-1 mRNA、HO-2 mRNA、HO-1和HO-2的表达水平。结果与C组比较，S组和SZ组血清Cr、BUN的浓度、尿α-M、RBP、NAG、T-GT水平、COHb、肾组织MDA、HO-1 mRNA、HO-1水平升高，肾组织SOD活性降低（P〈0．05），Z组上述指标差异无统计学意义（P〉0．05）；与S组比较，SZ组血清Cr、BUN的浓度、尿α1-M、RBP、NAG、γ-GT水平和肾组织MDA含量升高，肾组织SOD活性、COHb和肾组织HO-1mRNA、HO-1水平降低（P〈0．05）；4组大鼠肾组织／40-2mRNA、HO-2表达差异无统计学意义（P〉0．05）。结论肾组织HO—1对感染性休克大鼠的肾功能可产生一定的保护作用。Objective To investigate the role of heme oxygenase-1 （HO-1） in renal function injury during septic shock in rats. Methods Eighty healthy SD rats of both sexes, aged 3-4 months, weighing 260-330 g, were randomly divided into 4 groups （ n = 20 each） ： control group （ group C）, septic shock group （group S）, S ＋ ZnPP-Ⅸ group （group SZ） and ZnPP-Ⅸ group （group Z）. Group C received iv normal saline 0.5 ml and 2 h later,received intraperitoneal 50 mmol/L sodium bicarbonate 1 ml. Group S received iv LPS 10 mg/kg and 2 h later, received intraperitoneal 50 mmol/L sodium bicarbonate 1 ml. Group SZ received iv LPS 10 mg/kg and 2 h later, received intraperitoneal ZnPP-Ⅸ 10 μmol/kg. Group Z received iv normal saline 0.5 ml and 2 h later, received by intraperitoneal ZnPP-Ⅸ 10 μmol/kg. Carboxyhemoglobin concentration （COHb）, concentrations of senun urea nitrogen （BUN） and creatinine （Cr）, urinary α1 -microglobuliu （ α1 -M） concentration, N-acetyl-beta- D-glucosamidase （NAG） activity, retinol binding protein （RBP） concentration and 7-glutamyl transpeptidase （γ-GT） activity, MDA content, SOD activity and expression of HO-1 mRNA, HO-2 mRNA, HO-1 and HO-2 in renal tissue were detected at 4 h after administration of sodium bicarbonate or ZnPP-Ⅸ. Results The concentrations of serum BUN and Cr, urinary α1-M concentration, NAG activity, RBP concentration and T-GT activity, COHb, MDA content, expression of HO-1 mRNA and HO-1 in renal tissue were significantly higher and SOD activity lower in group S and SZ than in group C （ P 〈 0.05 ）, while there were no significant difference in the above parameters between group Z and C （P 〉 0.05）. The concentrations of sermn BUN and Cr, urinary α1-M concentration, NAG activity, RBP concentration and γ-GT activity, and MDA content in renal tissue were significantly higher, while SOD activity, COHb, and expression of HO-1 mRNA and HO-1 lower in group SZ than in group S （ P 〈 0.05）. There was no significant diff

关 键 词：休克 脓毒性 血红素氧化酶(脱环) 肾功能试验 

分 类 号：R459.7[医药卫生—急诊医学] R692[医药卫生—治疗学]