紫枝玫瑰的生物特性与组织培养研究  

Study on Biological Characteristics and Tissue Culture in Vitro of Rosa rugosa‘Purple Branch’

作　　者：田新华 张建瑛 王艳敏 李晶 张妍妍 

机构地区：[1]黑龙江省林业科学研究所速生林木培育重点实验室,黑龙江哈尔滨150081

出　　处：《北方园艺》2009年第6期48-50,共3页Northern Horticulture

基　　金：黑龙江省科技攻关资助项目(GB07B303-02)

摘　　要：以紫枝玫瑰腋芽为材料,研究了抑制外植体褐变的方法,筛选出分化增殖和生根的最佳培养基。分化增殖的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+ZT 0.1mg/L,增殖倍数达2.9倍,芽苗生长速度快,茎秆粗壮,叶片浓绿;最佳生根培养基为1/2MS+IAA 0.7mg/L,试管苗根系发达,生根数量多,生根速度快,有利于移栽成活。The methods for restraining the explant browning were studied with the axillary bud of Rosa rugosa ＇Purple Branch＇. The optimal culture medium for reproduction and rooting were chosen. MS medium supplemented with MS＋ 6-BA 1.0 mg/L＋NAA 0. 2 mg/L＋ZT 0. 1 mg/L was best for reproduction and multiplication can reach 2. 9 times after 35 days. The shoots grow fast with stem thick and leaf green. 1/2MS medium added IAA 0. 7 mg/L was best for rooting. The roots of test-tube seeding grow fast, quickly and abundantly, which were beneficial to the survival ratio of planting.

关 键 词：紫枝玫瑰 组织培养 培养基 

分 类 号：S685.12[农业科学—观赏园艺] S603.6[农业科学—园艺学]