山羊毛囊干细胞的分离及体外培养  

Isolation and in vitro culture of goat hair follicle stem cells

作　　者：史明艳[1] 刘霞[2] 杨学义[1] 窦忠英[3] 

机构地区：[1]洛阳师范学院生命科学系,河南洛阳471022 [2]延安大学生命科学学院,陕西延安716000 [3]西北农林科技大学国家干细胞工程技术研究中心陕西分中心,712100

出　　处：《黑龙江畜牧兽医》2009年第6期1-2,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30200137);国家“863”计划项目(2002AA216161);教育部重大科技专项项目(03160)

摘　　要：试验采用2．4U／mL Dispase酶消化和机械切割法分离毛囊隆突部，经胰酶（0．5mg／mL胰酶＋0．2mg／mL EDTA）消化从山羊耳部皮肤分离得到的毛囊干细胞，然后进行形态学观察、细胞生长曲线测定、克隆形成率及免疫组化染色检测，并对毛囊干细胞的基础培养基进行了筛选。结果表明：DMEM／F12是一种适合毛囊干细胞体外增殖培养的培养基；在以DMEM／F12为基础培养液的培养体系中细胞可传代至19代。Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0. 2 mg/mL EDTA. The resultant cells were cultured and studied by morphological observation, cell growth curve and colony-forming efficiency（ CFE）, and identified by, immunohistochemical staining. The results showed that DMEM/F12 was a good cell culture medium for goat hair follicle stem cells. And the data proved that the goat follicle stem cells can survive to the 19 subculture in vitro.

关 键 词：毛囊干细胞 毛囊隆突部 克隆形成率 体外培养 

分 类 号：S827[农业科学—畜牧学]