广西巴马小型猪1号染色体DNA文库的构建和鉴定  

Construction and identification of chromosome 1 DNA library of Guangxi Bama miniature swine

作　　者：孙雷[1] 路毅[1] 庄新杰[1] 曾有权[1] 张明[1] 陆阳清[1] 卢晟盛[1] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所广西亚热带生物资源保护利用重点实验室

出　　处：《黑龙江畜牧兽医》2009年第6期13-15,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：广西科技开发项目(桂科攻0330004-13)

摘　　要：研究利用玻璃针显微分离了巴马小型猪外周血淋巴细胞有丝分裂中期的1号染色体，然后将显微分离的染色体进行简并寡核苷酸引物PCR（DOP—PCR）扩增，通过PCR技术和荧光原位杂交（Florescence in situ hybridization，FISH）技术对扩增产物来源进行鉴定后，将DOP—PCR产物与pMD18-T载体连接、转化以构建1号染色体微克隆文库。结果表明：DOP—PCR扩增产物与猪的1号染色体DNA同源，并且得到了插入片段为100～600bp的巴马小型猪的1号染色体微克隆DNA文库。The chromosome 1 of the Guangxi Bama miniature swine was microdissected from the metaphase spreads of Bama miniature swine blood cells with a glass needle controlled by a micromanipulator. The DNA of chromosome 1 was amplified by DOP - PCR. After it was confirmed that the amplification DNAs came from chromosome 1 of swine by PCR and Florescence in situ hybridization （ FISH）, the PCR products were cloned into pMD18 - T vector. The results showed that the amplification DNAs came from chromosomel of swine. The chromosome 1 specific DNA library of Bama swine was established and analyzed. The insert size of the library was 100 bp to 600 bp.

关 键 词：猪 染色体微分离 DOP—PCR 染色体DNA 文库 

分 类 号：S813[农业科学—畜牧学]