慢性非细菌性前列腺炎／慢性盆腔疼痛综合征患者前列腺液中炎症因子差异表达的临床意义  

Clinical significance of differential expression of inflammatory factors in chronic non-bacterial prostatitis/chronic pelvic pain syndrome

作　　者：周青[1] 田雪飞[1] 袁轶峰[1] 袁博[1] 皮硕煌[1] 龚秀英[1] 王述湘[1] 徐华[1] 

机构地区：[1]湖南中医药大学第一附属医院中医外科,长沙410007

出　　处：《中华泌尿外科杂志》2009年第6期386-389,共4页Chinese Journal of Urology

基　　金：湖南省教育厅优秀青年资助项目（078056）;湖南省中医药科研基金资助项目（6206）

摘　　要：目的探讨慢性非细菌性前列腺炎／慢性盆腔疼痛综合征（CAP／CPPS）患者前列腺液（EPS）中炎症因子差异表达的临床意义。方法选取符合CAP／CPPS诊断标准的患者EPS标本38例（CAP18例，CPPS20例）．20例健康者EPS标本作对照。应用抗体芯片检测EPS中40种炎症因子的表达差异。结果与对照组比较，CAP患者7种炎症因子包括单核细胞趋化因子（MCP）1、可溶性肿瘤坏死因子受体Ⅱ（STNFRⅡ）、血小板衍生生长因子-BB（PDGF—BB）、白细胞介素（IL）-18、IL-11、IL-6、MCP-2表达增高了1．50倍以上，CPPS患者5种炎症因子包括MCP-1、PDGF-BB、MCP2、STNFRⅡ、IL-11表达增高了1．50倍以上；聚类分析结果表明，MCP1、PDGF—BB为最后聚合的炎症因子，CAP组中分别上调3．47、2．07倍．CPPS组中分别上调2．25、2．19倍；与CPPS组比较，CAP组IL-1β、MCP-1、STNFRⅡ表达分别上调1．85、1．55、1．67倍。结论CAP／CPPS发病过程中炎症因子表达明显增高．CAP的炎症反应程度高于CPPS，MCP1、PDGF—BB有可能成为CAP／CPPS诊断的炎症标志物。Objective To investigate the role of inflammatory cytokines in the pathogenesis of chronic non-bacterial prostatitis/ehronie pelvic pain syndrome （CAP/CPPS） patients. Methods The 38 cases with CAP/CPPS patients （18 cases of CAP and 20 cases of CPPS） and 20 cases of healthy controls were selected. The differential expressions of 40 kinds of inflammatory cytokines were detected by antibody arrays in prostate fluid. Results The inflammatory cytokines which increased more than 1.5 times expression have been found. There were seven kinds in CAP including monoeyte che moattractant protein （MCP）-1, solution tumor necrosis factor receptor Ⅱ（s TNF RⅡ ）, platelet-de rived growth factor-BB （PDGF BB）, interleukin （IL）- 1β, IL-11 ,IL 6,MCP 2 and five kinds in CPPS groups including MCP 1,PDGF-BB, MCP-2,s TNF R Ⅱ 、 IL-11 respectively, compared with healthy control group. The cluster analysis results showed that protein expression of Monocyte chemoattraclant protein 1 （MCP-1）and platelet-derived growth factor BB （PDGF BB） were significantly increased in CAP （3.47 and 2.07 times） and CPPS （2.25 and 2.19 times） compared with healthy control group and were the final polymerization of inflammatory eytokines. The protein expression of interleukin 1β （IL-1β）, MCP-1 and soluble tumor necrosis factor Ⅱ （s TNF R Ⅱ ） in CAP group was increased more than 1.85,1.55,1.67 times compared with CPPS group. Conclusions Elevated expression of inflammatory cytokines may play an important role in the course of CAP/CPPS disease. The extent of the inflammatory response of CAP was higher than CPPS. The inflammatory factors of MCP-1 and PDGF-BB could serve as a novel diagnostic marker.

关 键 词：抗体芯片 前列腺炎 炎症 细胞因子 

分 类 号：R686[医药卫生—骨科学]