芙蓉菊化学成分对体外培养大鼠胰岛分泌胰岛素作用的研究  

Effects of chemical constituents of Crossostephium chinense on insulin secretion in rat islets in vitro

作　　者：邹磊[1,2,3] 吴琦[1] 杨秀伟[1] 傅德贤[2] 

机构地区：[1]天然药物及仿生药物国家重点实验室北京大学药学院天然药物学系,北京100191 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100080 [3]中国医药研究开发中心有限公司,北京102206

出　　处：《中国中药杂志》2009年第11期1401-1405,共5页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2001CB51008)

摘　　要：目的:研究芙蓉菊化学成分在体外对培养的大鼠胰岛分泌胰岛素的作用。方法:体外培养大鼠胰岛,通过受试化合物作用前后胰岛分泌胰岛素水平的比较,考察芙蓉菊化学成分艾菊素、万寿菊黄素-3,6,7-三甲醚、5-O-甲基-myo-肌醇、东莨菪素和东莨菪苷对胰岛分泌胰岛素的作用,并与阳性对照药格列苯脲进行比较。结果:万寿菊黄素-3,6,7-三甲醚对胰岛分泌胰岛素的促进作用约为空白对照组分泌水平的2倍。5-O-甲基-myo-肌醇对胰岛分泌胰岛素亦有促进作用,约为空白对照组分泌水平的1.5倍。但是,东莨菪素对胰岛分泌胰岛素有抑制作用,约比空白对照低1.9倍。结论:对体外培养的大鼠胰岛分泌胰岛素功能,艾菊素、万寿菊黄素-3,6,7-三甲醚和5-O-甲基-myo-肌醇有促进作用;东莨菪素有抑制作用。Objective： To investigate the effects of the chemical constituents of the whole herbs of Crossostephium chinense on insulin secretion in rat islets. Method： Islets were isolated from rat pancreata, cultured in vitro, and measured by color signals of dithizone stained digestion solution for detection of pancreatic islets. The morphological observation of islets was carried out by inverted microscope. The effects of test compounds, scopoletin （ 1 ）, scopolin （ 2 ）, tanacetin （ 3 ）, quercetagetin-3,6,7-trimethylether （ 4） and 5-O-methyl-myo-inositol （5） isolated from the whole herbs of C. chinense, on the insulin secreting level from islets were compared with those of glybenclamide as a positive control substances, and the difference in insulin secreting level from islets between the presence and absence of test compounds was assayed. Result： There was no difference in basal insulin secretion before and after 2 h incubation period of rat islets. The islets treated with quercetagetin-3,6,7-trimethylether have about 2-fold higher insulin secreting level （P 〈 0. 01 ） compared a normal control group. The islets treated with 5-O-methyl-myo-inositol have about 1.5-fold higher insulin secre- ting level （P 〈 0. 05 ） compared to a normal control group. Whereas the islets treated with scopoletin show about 1.9-fold lower basal insulin secreting level （ P 〈 0. 05 ） than a normal control group. Conclusion： In this paper the developed cultivation method of isolated pancreatic islets from rat can be used as a kind of islet-based drug screening model for diabetes mellitus in vitro. Quercetagetin-3,6,7- trimethylether and 5-O-methyl-myo-inositol could enhance rat islet insulin secretion and further in vivo studies are needed to clarify the nature of such an observation. However, scopletin suppress rat islet insulin secretion.

关 键 词：芙蓉菊 万寿菊黄素-3 6 7-三甲醚 5—O-甲基-myo-肌醇 大鼠胰岛 胰岛素 

分 类 号：R285.5[医药卫生—中药学]