银屑病皮损表皮内Akt的激酶活性增强  

Increased activities of Akt in psoriatic epidermis

作　　者：张晓艳[1] 周平[3] 尤立平[1] 于长安[2] 潘琳[2] 马圣清[4] 

机构地区：[1]卫生部中日友好医院皮肤科,北京100029 [2]卫生部中日友好医院临床医学研究所 [3]北京世纪坛医院皮肤科 [4]北京大学第一临床医院皮肤科

出　　处：《中华皮肤科杂志》2009年第6期413-416,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30671885,30872266）

摘　　要：目的探讨Akt在银屑病发病中的意义。方法免疫组化、免疫印迹法与活性测定的方法对30例寻常型银屑病患者（进行期皮损和非皮损表皮）和20例正常人表皮中Akt的表达与磷酸化水平及Akt的活性分别进行检测，免疫染色的强度进行光密度测定，免疫印迹和激酶活性测定结果均采用灰度扫描，统计学处理采用方差分析和t检验。结果免疫组化显示：正常表皮、银屑病皮损和非皮损表皮内Akt蛋白的表达水平差异无统计学意义（Fakt=0．611，P〉0．05）；正常表皮与银屑病非皮损表皮内，磷酸化Akt的表达差异无统计学意义（Tp-akt=0．624，P〉0．05），与二者相比，银屑病皮损表皮内磷酸化Akt的表达明显增强（Fp-akt=19．081，P〈0．01）。免疫印迹的结果（Takt=1．378，P〉0．05，Tp-akt=237．75，P〈0．01）与免疫组化一致。与正常表皮相比，银屑病皮损表皮内Akt的活性增强（Tp-akt=138．441，P〈0．01）。结论银屑病角质形成细胞的过速增殖可能与银屑病皮损表皮内Akt的活性增强有关。Objective To investigate the significance of Akt in the pathogenesis of psoriasis. Methods Tissue specimens were obtained from involved and uninvolved skin of 30 patients with progressive psoriasis vulgaris and normal skin of 20 human controls. Immunohistochemistry, immunobloting and kinase activity assay were performed to detect the expressions of Akt and phosphorylated Akt as well as Akt activities in these specimens. Immunostaining intensity was assessed by optical density detection and the results of immunoblot and activity assay by grey scanning. Statistical analyses were performed by variance analysis and student＇s t test. Results As immunohistochemistry revealed, there was no significant difference in Akt protein expression among normal epidermis, psoriatic epidermis and uninvolved epidermis （F = 0.611, P 〉 0.05）; the level of phosphorylated Akt in psoriatic epidermis was significantly higher than that in normal epidermis and psoriatic uninvolved epidermis （F = 19.081, P 〈 0.01 ）, while no significant difference was observed between normal epidermis and psoriatic uninvolved epidermis （t = 0.624, P 〉 0.05 ）. Immunoblot showed a significant difference in phosphorylated Akt （t = 237.75, P 〈 0.01 ） but not in Akt （t = 1.378, P 〉 0.05 ） between psoriatic involved epidermis and normal epidermis. In comparison with normal epidermis, the activity of Akt in psoriatic involved epidermis was increased significantly （t = 138.441, P 〈 0.01 ）. Conclusion The overproliferation of psoriatic keratinocytes may be associated with increased activation of Akt.

关 键 词：银屑病 蛋白激酶B 免疫活性 

分 类 号：R758.63[医药卫生—皮肤病学与性病学] R735.1[医药卫生—临床医学]