RECK及MMP-14蛋白在神经母细胞瘤组织中的表达及与肿瘤转移关系的研究  

Expression of reversion-inducing cysteine-rich protein with Kazal motifs and matrix metalloproteinase-14 in neuroblastoma and researches on their roles in tumor metastasis

作　　者：于丹[1] 董蒨[1] 杨传民[1] 张虹[1] 江布先[1] 鹿洪亭[1] 郝希伟[1] 孙显路[2] 

机构地区：[1]青岛大学医学院附属医院小儿外科,266003 [2]青岛大学医学院附属医院病理科,266003

出　　处：《中华小儿外科杂志》2009年第6期341-344,共4页Chinese Journal of Pediatric Surgery

基　　金：本课题为国家自然科学基金资助（编号：30471800）及2007年青岛市科技发展计划（编号：072-1-5-nsh）资助课题

摘　　要：目的检测RECK蛋白及膜型基质金属蛋白酶MMP-14在神经母细胞瘤中的表达，探讨其在神经母细胞瘤的发生、发展、浸润和转移中的作用。方法选择临床及病理资料齐全的存档神经母细胞瘤蜡块标本36例，另取节细胞神经瘤蜡块标本10例作对照。采用第二代通用型二步法监测系统（PV-6000）免疫组织化学方法检测RECK及MMP14蛋白的表达。结果RECK蛋白在神经母细胞瘤组织中低表达（16．7％），并随肿瘤浸润深度的加深、远处转移的发生而降低（P=0．015〈0．05）。MMP-14在神经母细胞瘤组织中高表达（58．3％），并随肿瘤浸润深度的加深、远处转移的发生而增高（P=0．002〈0．05）。神经母细胞瘤组织中RECK与MMP-14蛋白的表达呈负相关（r=-0．418，P〈0．05）。结论神经母细胞瘤组织中RECK低表达，而MMP-14高表达。RECK及MMP-14可能成为评估神经母细胞瘤发生浸润转移的重要指标。Objective To detect the expression of RECK （reversion-inducing cysteine-fich protein with Kazal motifs）, MMP-14 （matrix metalloproteinase-14）, as well as their correlations in neuroblastoma and to investigate their roles in metastasis and development of the tumor. Methods PV-6000 immunohistochemistry was used to measure the expression of RECK and MMP-14, in 36 samples of neuroblastoma tissue selected from paraffin wax-embodied specimens with complete clinicopathological data, and another 10 cases of ganglioneuroma tissue Results The expression rate of RECK in neuroblastoma was low （16. 7%）, and reduced with the increased invasive depth and long-distance metastasis（P = 0. 015〈0. 05）. The expression rate of MMP-14 in neuroblastoma was high （58. 3%）, and elevated with the increased invasive depth and long distance metastasis （P = 0. 002%0. 05）. The expression of RECK was negatively correlated with the expression of MMP-14 （r = - 0. 418, P〈0. 05）. Concluions RECK is lowly expressed in neuroblastoma, but MMP-14 is highly expressed. RECK, MMP-14 may be served as the markers in the estimation of metastasis and dissemination of neuroblastoma.

关 键 词：神经母细胞瘤 基质金属蛋白酶类 免疫组织化学 

分 类 号：R686[医药卫生—骨科学]