PPARα和PPARγmRNA在2型糖尿病大鼠心肌中的表达及糖脂安的干预作用  

Study of the expression of PPARα、PPARγmRNA in myocardium of type 2 diabetic rats and the intervention effect of Tangzhi'an

作　　者：郑承红[1] 黄蓓 彭聪[1] 

机构地区：[1]武汉市中西医结合医院,湖北武汉430022 [2]孝感市中医院,湖北孝感432000

出　　处：《中国医院药学杂志》2009年第11期867-869,共3页Chinese Journal of Hospital Pharmacy

基　　金：武汉市卫生科研基金(编号:武卫[2007]43号)

摘　　要：目的:观察2型糖尿病大鼠心肌过氧化物酶体增殖激活物受体α(PPARα)、过氧化物酶体增殖激活物受体γ(PPARγ)mRNA的表达及糖脂安的干预作用。方法:尾静脉注射链脲佐菌素并高糖高脂饲料喂养诱导2型糖尿病大鼠模型。随机分为糖脂安组、二甲双胍组、模型组与正常组。采用逆转录聚合酶链反应(RT-PCR)法测定糖尿病大鼠心肌PPARα、PPARγmRNA的表达。结果:模型组心肌PPARα、PPARγmRNA表达较正常对照组明显降低(P<0.01)。药物治疗后,糖脂安组心肌PPARα、PPARγmRNA表达明显增高(P<0.01),其中,PPARγmRNA与二甲双胍组之间有统计学差异(P<0.05),PPARαmRNA与二甲双胍组之间有显著统计学差异(P<0.01)。结论:糖脂安能通过上调PPARα和PPARγmRNA的表达,具有类似PPARα/γ双重激动剂的作用。OBJECTIVE To observe the expression of peroxysome proliferator-activated receptor （PPARα） ,peroxysome pro liferator activated receptor γ（PPARγ） mRNA in myocardium of diabetic rats and the intervention effect of Tangzhi＇ an. METHODS Wistar rats were induced for diabetes by streptozotocin intravenous injection and treated with high-sugar-fat diet. then randomly devided into 4 groups： Tangzhi＇ an group, metformin group,model group and normal control group. The expres sion of PPARα and PPARγmRNA of myocardium were measured by reverse transcriptive polymerase chain reaction（RT- PCR）. RESULTS The expression of PPARα and PPARγmRNA of model group decreased（P〈0. 01 ）. After treatment, PPARα and PPARγmRNA expression of Tangzhi＇ an group increased （P〈0. 01 ）, and there were difference of PPARγmRNA （P〈0. 05） and ARαmRNA （P〈0. 01 ） between it and metformin group. CONCLUSION Tangzhi＇ an can increase the expression of PPARα and PPARγmRNA.possesses the PPARα /γ agonists effect.

关 键 词：糖脂安 2型糖尿痛 逆转录聚合酶链反应 过氧化物酶体增殖激活物受体α 过氧化物酶体增殖激活物受体Γ 

分 类 号：R963[医药卫生—微生物与生化药学]