北美海蓬子SbNHX1基因耐盐性及功能结构域分析  

Functional Domain and Salt-tolerance Analysis of SbNHX1 Gene from Salicornia bigelovii Torr.

作　　者：刘钢[1,2] 肖前谷[1] 段瑞军[1] 郭建春[1] 

机构地区：[1]中国热带农业科学院生物技术研究所 农业部生物技术重点实验室,海口571101 [2]海南大学农学院,海南儋州571737

出　　处：《西北植物学报》2009年第5期874-881,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点基础研究发展973计划(2007CB108903);中央级公益性科研院所基本科研项目(ITBBYB072)

摘　　要：对从北美海蓬子中分离的Na+/H+逆向转运蛋白基因SbNHX1进行了耐盐性及功能结构域分析.利用套叠PCR技术去除SbNHX1基因C末端162个核苷酸,得到SbNHX1-C基因,然后将SbNHX1、SbNHX1-C和拟南芥Na+/H+逆向转运蛋白基因AtNHX1分别插入pET22b(+)表达载体,转化大肠杆菌B菌株,进行各种金属盐离子胁迫分析.结果表明,北美海蓬子Na+/H+逆向转运蛋白基因SbNHX1只对Na+、K+离子有抗性,且耐盐性强于拟南芥Na+/H+逆向转运蛋白基因AtNHX1.缺失C末端的SbNHX1-C基因对Na+、K+离子胁迫无抗性,说明北美海蓬子Na+/H+逆向转运蛋白基因SbNHX1的耐盐作用与该基因C末端1353bp至1514bp的序列密切相关.In this report,we analyzed salt-tolerance and functional domain of Na^＋/H^＋ antiporter gene SbNHX1 separated from Salicornia bigelovii Torr.. SbNHX1 gene,the mutated SbNHX1-C gene,which cut off 162 bp at C-terminal of SbNHX1 by overlap extensing PCR,and Arabidopsis Na^＋/H^＋ antiporter gene AtNHX1 were inserted into the pET22b（＋） and transformed to E. coli B separately. The salt-tolerant of some alkali cation has been analyzed. The result indicated that SbNHX1 could only resist to Na^＋ and K^＋ , which the tolerance was higher than that of AtNHX1. SbNHX1-C gene,which deleted C-terminal, could not resist to Na^＋ and K^＋. It is deduced that the sequence from 1 353-1 514 bp in SbNHX1 gene plays an important role for SbNHX1 to Na^＋ and K^ tolerances.

关 键 词：北美海蓬子 Na+/H+逆向运输蛋白 盐胁迫 大肠杆菌 

分 类 号：Q789[生物学—分子生物学]