农杆菌介导Ds转座因子转化番茄群体的构建  

Agrobacterium Mediated Construction of Tomato Population with Ds Transposon

作　　者：陈双臣[1] 王凤华[1] 刘爱荣[1] 李君明[2] 

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《西北植物学报》2009年第5期882-887,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30700002);河南省自然科学基金(2008B210002,2009B210004);河南科技大学校基金(2007QN029)

摘　　要：以番茄Micro-Tom子叶为试材,绿色荧光蛋白GFP基因为报告基因,利用农杆菌介导的遗传转化法将构建的激活表达标签pAcGFP导入番茄Micro-Tom,获得转基因番茄群体.对转化群体进行了GFP基因活性及分子检测,结果表明,GFP已经整合到番茄基因组中.以GFP基因设计引物进行PCR检测表明,阳性率为91.43%.通过Southern杂交分析,31.25%的个体为一个插入位点,56.25%为2个插入位点,T-DNA在基因组中的平均拷贝数为1.8个.The activation tagging pAcGFP was transformed into tomato via Agrobacterium with GFP as reporter gene. The transformed tomato population was gained. GFP and molecular detection were conducted with the transformed plants. The results showed that GFP gene was interknitted into tomato genome. Analyzed with Southern blotting,about 31.25 % transgenic plants among T1 population have one inserted site and 56.25% with two inserted sites,the average inserted T-DNA sites were 1.8.

关 键 词：番茄 激活标签 绿色荧光蛋白基因 遗传转化 

分 类 号：Q789[生物学—分子生物学]