焕镛木小孢子发生及雄配子体发育研究  

Microsporogenesis and Male Gametophyte Formation of Woonyoungia septentrionalis(Dandy) Law

作　　者：谭金桂[1] 吴鸿[2] 李勇[1] 张寿洲[1] 

机构地区：[1]深圳仙湖植物园,广东深圳518004 [2]华南农业大学生命科学学院,广州510642

出　　处：《西北植物学报》2009年第5期937-944,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：深圳市城管局科研项目

摘　　要：运用常规石蜡切片法和细胞学压片法,对焕镛木[Woonyoungia septentrionalis(Dandy)Law]小孢子发生、雄配子体发育以及花粉萌发进行观察分析.结果显示:焕镛木花单性,雄花的雄蕊多数、离生,每一花药具4个花粉囊,花药壁5～7层.腺质绒毡层具1～2层细胞;小孢子形成时胞质分裂方式为修饰性同时型;四分体排列方式以四面体型为主,并有交叉型和左右对称型,偶见T型和直线型;成熟花粉粒为二细胞型花粉粒.小孢子发生和雄配子体发育过程中存在变形及败育现象.花粉萌发率为79.89%～80.62%.结合焕镛木花粉粒数目和萌发率的分析认为,雄配子体发育中的败育不是影响焕镛木濒危的主要因素.The formation of microspore, development of male gametophyte and germination rate of pollen grains in Woonyoungia septentrionalis （Dandy） Law were observed by serial paraffin section technique and cytology compression method. The results are as follows：each stamen has 4 sporangia and the anther wall is composed of 5-7 layers,including epidermis,fibrous endothecium,middle layer （2-3 layers） and glandular tapetum （1-2 layers）. Cytokinesis at the meiosis of microspore mother cell is modified simultaneous type,and tetrads are tetrahedral, isobilateral or decussate, occasionally linear and T type. Mature pollen grains are released at 2-celled stage. The results showed that few abnormal phenomenon appeared at each stage in the process of microsporogenesis and pollen development. The artificial germination rate of pollen grains was 79.89-80.62% ,which suggested that the abnormity in the process of male gametophyte formation was not the main factor which lead to the low seed setting rate.

关 键 词：焕镛木 小孢子发生 雄配子体发育 

分 类 号：Q944.58[生物学—植物学]