钙离子及钙调蛋白对不同温度型冬小麦盐分吸收与累积的影响  

Function of Calcium Ion and Calmodulin on Sodium Uptake and Accumulation of Winter Wheat with Different Canopy Temperature

作　　者：李青松[1] 王林权[1] 周春菊[2] 王俪梅[1] 尚浩博[1] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨陵712100 [2]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北植物学报》2009年第5期975-982,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30571085)

摘　　要：以4种不同温度类型的冬小麦品种小偃6号、NR9405、陕229和RB6为材料,在0～250mmol/LNaCl胁迫条件下,采用水培试验研究了钙调蛋白抑制剂(CPZ)、钙离子通道阻断剂(LaNO3)和钙离子螯合剂(EGTA)等对冬小麦盐分吸收的影响及其作用机理.结果显示:在中盐(150mmol/LNaCl)胁迫下3种制剂均显著增加了各品种小麦植株对Na+的吸收,减少了K+吸收,且其作用依次表现为CPZ>EGTA>LaNO3.中盐胁迫下,各种制剂对冷型小麦陕229和RB6盐分吸收的影响大于暖型小麦小偃6号和NR9405,而高盐(250mmol/L)胁迫下这种差异缩小.研究表明,Ca2+对小麦Na+吸收的调节作用主要通过钙调蛋白(CaM)来完成,CaM不但控制小麦Na+净吸收和累积,而且在小麦K+吸收中也起重要作用;在盐胁迫下冷型小麦陕229和RB6对Ca2+和CaM活性的要求大于暖型小麦小偃6号和NR9405.Under 0-250 mmol/L NaCl concentrations, hydroponic experiments were conducted to investigate the effect of calmodulin inhibitor （chlorpromazine,CPZ）, Ca^2＋ channels inhibitor （LaNO3） and Ca^2＋ chelator （EGTA） on sodium and potassium uptake and its＇ mechanism with 4 winter wheat varieties, ＇Xiaoyan 6＇, ＇NR9405＇, ＇Shaan 229＇ and ＇RB6＇ ,which are different in canopy temperature. The results indicated that under medium （150 mmol/L,NaCl） salt concentration, sodium uptake is promoted significantly, and the potassium uptake is slowed down by 3 inhibitors. And the effect of inhibitors is as follow：CPZ〉 EGTA〉LaNO3. So the function of Ca^2＋ on sodium uptake mainly by way of combination of Ca^2＋ and calmodulin （CAM）. The calmodulin not only regulate Na^＋ efflux,which is critical to net Na^＋ influx in root cells and accumulation in the shoot, but also regulate K^＋ influx. Under medium salt concentration （150 mmol/ L,NaCl）,the effect of inhibitors or chelator on the Na^＋ uptake of ＇Shaan 229＇ and ＇RB6＇ is higher than that of ＇Xiaoyan 6 ＇ and ＇NR9405 ＇ , but there is little difference among the different genotypes under higher salt stress （250 mmol/L NaCl）. So that ＇Shaan 229＇ and ＇RB6＇ are more sensitive to lower Ca^2＋ and CaM activity than that of ＇Xiaoyan 6＇ and ＇ NR9405 ＇.

关 键 词：冬小麦 盐胁迫 钙离子 钙调蛋白 

分 类 号：Q945.78[生物学—植物学]