人脐血间充质干细胞移植促进大鼠颅脑创伤后神经功能的恢复  

HUCB-MSCs transplants promote neurological functional recovery after traumatic brain injury

作　　者：陈镭[1] 惠国桢[2] 张赛[1] 苗宗宁[3] 孙洪涛[1] 胡群亮[1] 刘振林[1] 

机构地区：[1]武警医学院附属医院脑系科中心,天津300162 [2]苏州大学第一附属医院神经外科 [3]无锡市第三人民医院中心实验室

出　　处：《中华创伤杂志》2009年第6期498-502,共5页Chinese Journal of Trauma

基　　金：基金项目：天津市科委重点攻关科研基金资助项目（06YFSF01200）;武警医学院科研基金资助项目（WBS200815）

摘　　要：目的将人脐带血（human umbilical cord blood，HUCB）间充质干细胞（mesenchymal stem cells，MSCs）注射到创伤性脑损伤（TBI）大鼠顶叶皮层及海马区，观察神经细胞标志物的表达和促进神经功能的恢复。方法HUCB—MSCs用双苯酰亚胺（bis—benzimide）标记24h，通过立体定向将细胞注射到损伤脑组织内。全部大鼠分为假损伤组、损伤组（TBI）、对照组（TBI＋PBS）和治疗组（TBI＋MSCs）。用免疫组化和免疫荧光标记的方法检测移植细胞的存活、迁移和分化情况，用神经损伤严重程度评分（NSS）对神经功能恢复结果进行评价。结果在接受移植的脑创伤大鼠局部有大量的MSCs存活，一些细胞分化为神经元或神经胶质细胞，并表达NSE和GFAP等神经细胞的标志物，治疗组的神经功能评分较其他组有明显的改善。结论HUCB—MSCs可明显改善脑创伤后神经功能的恢复，并能很好替代骨髓MSCs而用于干细胞移植。Objective To locally inject human umbilical cord blood （HUCB） mesenchymal stem cells （MSCs） to rat traumatic brain injury （TBI） model to investigate expression of neural markers and neurological functional improvement. Methods HUCB-MSCs were labeled by bis-benzimide for over 24 hours and stereotactically transplanted into the brain of the rats. All rats were divided into four groups, ie, sham injury group, TBI group, control （TBI ＋ PBS） group and treatment （TBI ＋ MSCs） group. Immunohistochemical methods and immunofluorescence staining were used to observe the survival, migration and differentiation of the transplanted cells. The neurological functional improvement was evaluated by using the neurological severity score （NSS）. Results There existed a large number of MSCs survived in local region of the brain that received transplants, when some MSCs differentiated into neurons or astrocytes and expressed the neurocyte markers including NSE and GFAP around the grafted site. Treatment group had significantly improved scores compared with sham injury group, TBI group and control group. Conclusions HUCB-MSCs transplantation can potentially improve neurological functional after TBI and may be a good alternative to bone marrow cells for stem cell transplantation or cell therapy.

关 键 词：脑损伤 脐带血 间充质干细胞 移植 

分 类 号：R651.1[医药卫生—外科学]