颅脑外伤后亚低温治疗对Calpain表达的影响  

Calpain expression changes in response to hypothermia after traumatic brain injury

作　　者：万杰清[1] 贾锋[1] 毛青[1] 江基尧[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,200127

出　　处：《中华创伤杂志》2009年第6期507-509,共3页Chinese Journal of Trauma

基　　金：基金项目：国家基础研究与发展规划资助项目（2005CB522600）;国家自然科学基金资助项目（30571908）

摘　　要：目的探讨亚低温治疗对颅脑外伤后Calpain基因和蛋白表达的影响。方法将27只SD大鼠按随机数字表法分为正常对照组、常温脑损伤组和亚低温脑损伤组。脑损伤组分别接受右顶部侧方中度液压损伤打击，其中亚低温组在伤后即刻接受持续4h的亚低温治疗[肛温（32±0．5）℃]。在伤后6，12，24和72h四个时间点分别通过荧光PCR、Westernblot定量检测各组打击伤灶周围皮层钙蛋白酶Calpain基因转录和蛋白分子表达的水平，同时运用免疫荧光观察Calpain蛋白分布情况。结果与常温组比较，亚低温使颅脑外伤后12h及24hCalpain基因的转录较正常对照和同时间点常温脑损伤组显著增加（P〈0．05）。然而，伤后6，12，24和72h亚低温治疗均使Calpain在蛋白水平较同时间点常温脑损伤组表达升高的程度显著抑制（P〈0．05）。结论颅脑外伤后亚低温治疗的脑保护机制可能与其在Calpain基因转录后明显抑制Calpain蛋白水平的表达有关。Objective To determine the effect of hypothermia on gene transcription and protein expression of calpain after traumatic brain injury （TBI）. Methods Twenty-seven rats were randomly divided into three groups, ie, normal control group, normothermia TBI group and hypothermia TBI group. All rats with TBI were suffered from a lateral fluid percussion injury （FPI） at the right parietal lobe. Hypothermia intervention [ rectal temperature for （ 32±0.5）℃] 3 was performed for four hours immediately after TBI in hypothermia TBI group. Fluorescence PCR and Westem blot were utilized to semi-quantify gene transcription and protein expression of calpain and immunofluorescence used to observe protein distribution of Calpain. Results Compared with normothermia TBI group and normal control group, hypothermia TBI group showed increased calpain gene transcription at 12 and 24 hours respectively after FPI （P 〈 0.05 ）. However, the increase of calpain protein expression in hypothermia TBI group was inhibited more significantly by hypothermia at 6,12,24 and 72 hours after TBI, compared with normothermia TBI group （ P 〈 0.05 ）. Conclusion Neuroprotection of hypothermia after TBI may somewhat be related to the decrease of calpain protein expression after its gene transcription.

关 键 词：脑损伤 低温 基因表达 CALPAIN 

分 类 号：R651.15[医药卫生—外科学]