性激素对狼疮模型鼠Th1／Th2类细胞因子分泌的影响  

Influence of Sexual Hormones on the Expression of Thl/Th2 Cytokines in MRL/lpr Lupus Model Mice

作　　者：邵松[1] 刘义庆[1] 许丽[2] 张炳昌[1] 崔相法[1] 陈国建[1] 卢志明[1] 

机构地区：[1]山东大学附属省立医院,济南250021 [2]山东大学第二医院,济南250033

出　　处：《现代检验医学杂志》2009年第3期31-33,共3页Journal of Modern Laboratory Medicine

基　　金：山东省卫生厅（2001CA20BA1）;山东省自然科学基金（Y2006C116）.

摘　　要：目的研究性激素对MRL／lpr狼疮模型鼠Th1／Th2类细胞因子表达的影响，探讨其对Th1／Th2平衡的调节作用。方法将狼疮模型鼠去势后随机分为空白对照组、雌二醇（E2）生理水平组、雌二醇（E2）妊娠水平组、睾酮（T）组。利用RT—PCR方法检测模型动物血液单个核细胞中Th1类细胞因子（IFN-γ，IL-2）、Th2类细胞因子（IL-4，IL-6）的基因转录状态。结果雌二醇生理水平组和妊娠水平组IL-2 mRNA的水平低于空白对照组（P〈0．05），雌二醇妊娠水平组IL-4和IL-6的mRNA水平高于空白对照组（P〈0．05）。睾酮组IL-6mRNA州平低于空白对照组（P〈0．05）。结论性激素可以调节Th1／Th2细胞因子的表达水平，妊娠剂量的雌激素使狼疮模型剥的细胞因子向Th2偏移，而雄激素则抑制Th2偏移。Objective To study the influences of sexual hormones on the expression of Th1/Th2 cytokines in MRL/lpr lupus model mice and investigate it＇s modulation on Thl/Th2 balance. Methods The ovariectomized lupus model mice were divided into blank control group,estradiol （E2） group of physiologic level ,estradiol（E2） group of gestational level,testosterone（T） group. Thl cytokines （IFN-Y, IL-2） and Th2 cytokines （IL-4,IL-6） in peripheral blood mononuclear cell （PBMC） from the animal model were detected by RT-PCR. Results The level of IL-2 mRNA in estradiol（E2） group of physiologic level and gestational level were significantly lower than that of blank control group（P〈0. 05）. The level of IL- 4 and IL-6 mRNA in estradiol（E2） group of gestational level were significantly higher than that of blank control group（P〈0. 05）. The level of IL-6 mRNA in testosterone（T） group was significantly lower than that of blank control group（P〈0. 05）. Conclusion Sexual hormones can modulate the expression levels of Th1/Th2 cytokines. Estradiol of gestational dosage can make the cytokines of lupus model mice Th2 excursion,however androgen can repress it.

关 键 词：雌二醇 系统性红斑狼疮 TH1/TH2细胞因子 

分 类 号：R593.24[医药卫生—内科学] R-332[医药卫生—临床医学]