M蛋白对氧化酶法测定血清胆红素的干扰及排除  

Interference by M Protein in the Enzymatic Method for Bilirubin Determination

作　　者：赵兴波[1] 李京华[1] 李江[1] 鄢盛恺[1] 

机构地区：[1]卫生部中日友好医院检验科,北京100029

出　　处：《现代检验医学杂志》2009年第3期99-101,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血清中M蛋白对氧化酶法测定胆红素产生干扰的可能原因。方法通过比较反应曲线，观察反应过程，分析试荆成分及反应环境，以及用其他方法测定，探讨干扰产生的原因。结果氧化酶法测定富含M蛋白血清胆红素出现异常反应曲线，样本与试剂混合产生白色混浊，将患者血清稀释后不能完全消除干扰，应用其他测定方法验证了干扰的存在。结论某些血清中高浓度的M蛋白能干扰胆红素氧化酶法的测定，应引起日常检验工作者的重视。Objective To investigate the possible mechanism of interference in the enzymatic method for bilirubin determination by M proteins in serum. Methods The causes of the interference were analyzed by studying on the reaction ab-sorbance curves,analyzing the reagent components and its reaction condition,and comparing the results with that of other method for bilirubin determination. Results There were abnormal reaction absorbance curve caused by M proteins in serum for bilirubin assays. And a white precipitate formed in the reaction mixtures. The interference couldn＇t be eliminated by dilutioning the serum. Conclusion Some serum contained rich M proteins could interfere with the enzymatic method for bilirubin determination. It suggested that laboratory staff should pay attention to this phenomenon in daily work.

关 键 词：M蛋白 干扰 胆红素 

分 类 号：R446.112[医药卫生—诊断学]