差异磁分离酶联免疫法检测血清甲胎蛋白的研究  

Studies on Differential Magnetic Enzyme Immunoassay for Detection of Alpha Fetoprotein in Serum

作　　者：徐兵[1] 陈纯美 徐如梅[3] 张建钫[3] 伍严安[3] 

机构地区：[1]福州市传染病医院肝病研究所,福州350025 [2]成都市维新生物技术公司,成都610061 [3]福建省立医院检验科,福州350001

出　　处：《现代检验医学杂志》2009年第3期109-111,共3页Journal of Modern Laboratory Medicine

摘　　要：目的改进磁分离酶联免疫法（MEIA）。方法采取预加微量标本、将磁铁置于微孔侧壁和扣除上清液A值法，改进MEIA建立了差异磁分离酶联免疫法（DMEIA）。求DMEIA回归方程、线性范围、变异系数（CV），比较DMEIA和全自动电化学发光法（ECLIA）配对检测100份血清AFP结果差异。结果DMEIA检测AFP的回归方程Y=3．94X＋16．45，r=0．992，线性范围5～500ng／ml，批内CV=7．7％，批间CV一8．50A；DMElA和ECLIA测定血清AFP值差异无统计学意义（f检验，P〉0．05）；检测原倍高浓度AFP时（21460～501690ng／ml）两法均未出现hook效应。结论DMEIA可基本克服hook效应，可用普通微孔板和酶标仪比色，可省去洗涤，检测AFP结果接近ECLIA，但线性范围小于ECLIA。Objective To improve the one-step sandwich magnetic enzyme immunoassay（MEIA）. Methods By pre-adding micro-quantity of a sample,putting the magnetic bar beside the micro-wells,deducting the A values of partial superanant, the differential magnetic enzyme immunoassay（DMEIA） was established. 100 serum samples were tested for quantitative analysis of AFP by DMEIA and the electric chemiluminescent immunoassay（ECLIA）. Results On detection of AFP by DMEIA,the regression equation was Y=3.94＋16.45,r=0. 992 ,the linear scope was 5-500 ng/ml,the inter and intra coefficients of variation （CV） were 7.70/00 and 8. 5 % respectively. The difference between the results of compared detections by DMEIA and ECLIA showed no statistic significance （t test,P〉0.05）. On detecting the high concentration （21 460-501 690 ng/ml） of AFP,the DMEIA did not show the hook effect. Conclusion The DMEIA could save the rinse,overcome the hook effect,be suitable for general EIA instruments and closed the results of ECLIA on detecting AFP,but the linear scope is smaller than the ECLIA.

关 键 词：酶免疫检测 磁分离 AFP 电化学发光法 HOOK效应 

分 类 号：R392-11[医药卫生—免疫学]