RGD多肽接枝聚复合导管桥接神经缺损的实验研究  

An experimental study of RGD peptide conjugated poly[ LA-（Gic-Lys） ] nerve conduit to bridge nerve gap

作　　者：黄继锋[1] 征华勇[2] 李世普[3] 严琼娇[3] 徐永年[1] 陈庄洪[1] 

机构地区：[1]武汉市广州军区武汉总医院骨科,湖北430070 [2]南方医科大学研究生学院,广州 [3]武汉理工大学生物材料中心

出　　处：《中华手外科杂志》2009年第3期154-157,共4页Chinese Journal of Hand Surgery

基　　金：国家重点基础研究发展计划资助项目（2005CB623905）;全军医药卫生科研基金（06MB165）

摘　　要：目的探讨RGD多肽接枝聚／β-TCP／PLA复合神经导管桥接周围神经缺损的治疗效果。方法45只雄性成年Wister大鼠，随机分为3组，每组15只。切断右侧坐骨神经形成10mm缺损，A组采用单纯PLA神经导管桥接缺损，B组采用RGD多肽接枝聚／β-TCP／PLA复合神经导管桥接缺损，C组采用自体神经移植。术后12周进行大体观察、电生理、小腿三头肌恢复率、组织学、超微结构等测定。结果B组运动神经传导速度和肌肉湿重恢复率明显优于A组，差异有统计学意义（P〈0．05）；B组与C组相近，差异无统计学意义（P〉0．05）。组织学、超微结构测定发现B、C组神经再生情况明显优于A组。结论在坐骨神经损伤修复中，RGD多肽接枝聚／β-TCP／PLA复合神经导管桥接修复效果与自体神经移植相近，可作为一种较理想的神经缺损修复材料。Objective To investigate the effect of RGD peptide conjugated poly[LA-（Glc-Lys）]/β- TCP/PLA nerve conduit for bridging peripheral nerve regeneration defect. Methods Forty-five male Wister rats were randomly divided into 3 groups, with 15 rats each. A 10 mm defect was created in the right sciatic nerve. In group A the gap was bridged by PLA tube. In group B RGD peptide conjugated pely[LA-（Glc-Lys） ]/ β-TCP/PLA nerve conduit was used to repair the defect. Autologous nerve graft was done in group C which served as control. Twelve weeks postoperatively nerve regeneration was evaluated by gross observation, electrophysiology, muscle weight and muscle morphometry of triceps surae, and uhrastructure of the regenerating nerve. Results Twelve weeks after the operation, nerve conduction velocity and muscle weight recovery of group B were better than those of group A. The differences were statistically significant（ P 〈 0.05）. There was no significant difference between group B and group C （ P 〉 0.05）. The results of histology and ultrastructure showed that nerve regeneration in group B and group C was significantly superior to that in group A. Conclusion RGD peptide conjugated poly[ LA-（Glc-Lys） ]/β-TCP/PLA conduit can achieve similar results in repairing sciatic nerve defect to that of autogentic nerve graft. It may be an ideal material to repair nerve defect.

关 键 词：周围神经 神经再生 RGD多肽接枝聚 

分 类 号：R686[医药卫生—骨科学]