β-神经生长因子缓释系统对原代培养鸡胚背根神经节轴突生长的影响  

The effects of β-NGF controlled delivery system on growth of primary cultured chick embryo dorsal root ganglion axons

作　　者：李学东[1] 邓志云 陈斌[1] 郑创义[1] 刘钊勇[1] 熊华花[3] 杜世新[1] 

机构地区：[1]汕头大学医学院第一附属医院骨科,广东515041 [2]广东省边防武警总队医院外科 [3]深圳市第二人民医院超声科

出　　处：《中华手外科杂志》2009年第3期161-164,共4页Chinese Journal of Hand Surgery

摘　　要：目的制备β-神经生长因子（nerve growth factor，NGF）缓释系统，评价其及其组份对体外培养鸡胚背根神经节（dorsal root ganglion，DRG）轴突生长的生物学效应。方法构建含pNGF50、100、250μg/L的缓释系统分别与DRG共同培养，DRG常规培养作为对照组，了解各浓度组DRG的轴突生长；分别利用缓释系统各组份与DRG培养，观察各组轴突生长情况。实验分成6组：对照组（DRG＋纤维蛋白原），A组（DRG＋纤维蛋白原＋肝素结合肽＋肝素钠＋100μg／Lβ-NGF），B组（DRG＋纤维蛋白原＋肝素钠＋100μg／Lβ-NGF），C组（DRG＋纤维蛋白原＋肝素结合肽＋100μg／Lβ-NGF），D组（DRG＋纤维蛋白原＋100μg／Lβ-NGF），E组（DRG＋纤维蛋白原＋肝素结合肽＋肝素钠）。结果50、1130和250μg／Lβ-NGF缓释系统轴突生长是对照组的1．31（P〉0．05）、3．78（P〈0．01）和3．05（P〈0．01）倍；100μg／L组较250μg/L组DRG轴突生长明显（P〈0．05）。实验组A、B、C、D和E组的DRG轴突生长分别是对照组的3．75、1．15、1．12、1．10和1．09倍，与对照组比较仅A组差异有统计学意义（P〈0．01）。结论β-NGF缓释系统中释放的β-NGF具有生物活性，可明显促进DRG轴突生长。Objective To construct β-NGF controlled delivery system and evaluate the biological effects of β-NGF on the growth of chick embryo dorsal root ganglion （DRG） axons in vitro. Methods Delivery systems releasing β-NGF at 50 μg/L, 100 μg/L and 250μg/L concentration were constructed. To determine the optimal dose response effects of NGF in the controlled delivery system, DRG were co-cultured with of β-NGF at above concentrations while using DRG basic culture as control. Axonal growth was observed. DRG were also co-cultured with the components in the controlled delivery system to detect the effects on growth of DRG axons. The experiment was divided into 5 experimental groups and 1 control group： control group, DRG ＋ fibrin; Group A, DRG＋ fibrin＋ peptide ＋ heparin ＋ 100μ/L β＋NGF; Group B, DRG＋ fibrin＋ heparin ＋ 100μg/L β-NGF; Group C, DRG ＋ fibrin ＋ peptide ＋ 100 μg/L β-NGF; Group D, DRG ＋ fibrin ＋ 100 μg/L β-NGF; Group E, DRG ＋ fibrin ＋ peptide ＋ heparin. Results The growth of DRG axons in 50 μg/L, 100μg/L and 250μg/L concentration of β-NGF controlled delivery system was 1.31 （ P 〉 0.05）, 3.78 （ P 〈 0. 01 ） and 3.05 （ P 〈 0.01） folds of the control respectively. The growth of DRG axons in 100 μg/L group was significantly better comparing to that in 250μg/L group. The grouh of DRG axons in Groups A, B, C, D and E was 3.75, 1.15, 1.12, 1.10 and 1.09 folds of the control group, respoctively. The difference was only statistically significant between Group A and the control group （ P 〈 0.01 ）. Conclusion β-NGF released from the β-NGF controlled delivery system was bioactive. It could promote the growth of DRG axons.

关 键 词：神经节 脊 神经生长因子 鸡胚 

分 类 号：R686[医药卫生—骨科学]