贵州白香猪的遗传检测  

Genetic Detection of Guizhou University White Xiang Pig

作　　者：于冰[1] 刘若余[1] 刘培琼[1] 

机构地区：[1]贵州大学动物科学学院省动物遗传育种与繁殖重点实验室,贵州贵阳550025

出　　处：《家畜生态学报》2009年第3期10-13,共4页Journal of Domestic Animal Ecology

基　　金：贵州省农业厅地方畜禽保护项目资助

摘　　要：选取27个微卫星位点,应用PCR技术对贵州白香猪的2个品系进行遗传质量监测。结果在所选的27个微卫星位点中,共有24个位点获得稳定的扩增结果,其中SW911位点与S0026两个位点在群体内表现为单态纯合。2个品系有效等位基因、平均多态信息含量、平均杂合度、基因纯合率和平均近交系数分别为1.8310、1.7951、0.3436、0.3249、0.4329、0.4188、52.63%、58.55%、0.5377、0.5605。结果表明微卫星标记可用于检测贵州白香猪的遗传质量,也可用于分析封闭群的遗传多态性。是一种有潜力的试验动物遗传学检测标记。27 microsatellites loci were selected to monitor the genetic quality in two strains Guizhou U- niversity white Xiang Pig by PCR. Results 24 microsatellites DNA could be amplified efficiently. It dis- played homozygosis in SW911 and S0026 loci. Results showed that： the number of effective allele , PICs , the gene heterozygosity, the gene homogeneity index and inbreeding coefficients of two breeds strains were 1. 8310,1. 7951;0. 3436,0. 3249;0. 4329,0. 4188;52. 63%,58. 55%and 0. 5377,0. 5605,respectively. The microsatellite marker is a potential marker for Guizhou University White Xiang Pig the genetic quality of laboratory animals.

关 键 词：贵州白香猪 微卫星 遗传检测 

分 类 号：S811.5[农业科学—畜牧学]