成熟脂肪细胞中脂联素基因表达的脂肪酸应答调控  

Response of Fatty Acids to Adiponectin Expression in Matured Adipocytes

作　　者：袁继红[1] 龙欢[1] 雷霆[1] 陈小冬[1] 杨在清[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070

出　　处：《华中农业大学学报》2009年第3期320-325,共6页Journal of Huazhong Agricultural University

基　　金：国家"973"项目(2006CB102100);国家"863"项目(2008AA10Z134);国家自然科学基金项目(30771585)资助

摘　　要：用不同种类、不同浓度游离脂肪酸(FFA)处理体外培养的成熟3T3-L1脂肪细胞,实时荧光定量PCR和Western-blotting分析FFA处理前后脂联素基因mRNA和蛋白质水平的表达差异。结果表明:各类脂肪酸对脂联素mRNA的表达均具有下调作用,且抑制效应呈时间、剂量依赖性;并且各种脂肪酸显著地影响脂联素蛋白的分泌作用。3T3-L1 preadipocytes were cultured and differentiated into the matured adipocytes in vitro. The differentiated adipocytes were incubated with different types of free fatty acids at different concentrations. Real-time quantitative PCR and Western-blotting were used to detect the expression of adiponectin mRNA and protein, respectively. The results showed that all types of fatty acids inhibitted adiponectin mRNA expression in dose and time-dependent manner. And all the fatty acids significantly influenced the secretion of adiponectin protein.

关 键 词：脂肪酸 脂联素 基因表达 脂肪细胞 

分 类 号：Q786[生物学—分子生物学]