口服脂质体包裹Ag85A DNA疫苗诱导抗体的产生  

Preparation of Liposome Coated Ag85A DNA Oral Vaccine and the Production of the Induction Antibody

作　　者：王大南[1] 刘滢[1] 张佩[1] 冯永辉[1] 单风平[1] Seif S.Salum Mchenga 吕昌龙[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳辽宁110001

出　　处：《微生物学杂志》2009年第2期39-42,共4页Journal of Microbiology

基　　金：国家自然科学基金项目(30571719)

摘　　要：制备脂质体包裹的Ag85A口服DNA疫苗,并观察小鼠口服后所诱生的抗体产生情况。用脂质体包裹重组质粒pcDNA3.1/myc-HisA-Ag85A制备口服DNA疫苗,并用脂质体包裹空质粒pcDNA3.1/myc-HisA作为对照。将C57BL/6小鼠随机分为3组,即生理盐水组、空质粒组和重组质粒DNA疫苗组。分别将生理盐水、空质粒和重组质粒DNA疫苗以灌胃方式投给各组小鼠,共免疫3次,每次间隔14d,末次免疫后14d处死小鼠,ELISA法检测血清中Ag85A特异性抗体水平,放射免疫法测定肠组织中分泌型IgA(sIgA)含量。重组质粒组血清中Ag85A特异性抗体滴度为1160,空质粒组和生理盐水组血清中均未捡出Ag85A特异性抗体。重组质粒组肠组织中sIgA含量(0.3761±0.0456)μg/mL较空质粒组(0.2374±0.0414)μg/mL和生理盐水(0.1993±0.0899)μg/mL组显著增高(P<0.05),而空质粒组和生理盐水组未见有意义的变化(P>0.05)。口服脂质体包裹Ag85ADNA疫苗可诱导外周特异性抗体的产生和肠道黏膜局部sIgA的水平的升高。Liposome was used to encapsulate recombinant plasmid pcDNA3.1/myc-HisA-Ag85A to prepare oral DNA vaccine; on the other hand an empty plasmid peDNA3.1/myc-HisA was encapsulated with liposome as a control. C% ＆ BL/6 mice were randomly divided into three groups： normal saline group, empty plasmid group, and recombinant DNA vaccine group. Respectively to each group of mouse fed with three preparations through intragastrie administration. The mice were immunized every 14 days, three times altogether. 14 days after the latest administration, the mice were sacrificed and determined serum antibody against Ag85A by Elisa and determined slgA in the gut by ratio-immunoassay. The results showed that the level of specific anti-Ag85A antibody for recombinant plasmid group was 1 ： 160, and no anti-Ag85A antibody was detected out for either empty plasmid nor normal saline control group, slgA level in the gut for recombinant plasmid group is （0. 199 3 ± 0. 089 9） μg/mL, significantly increased as compared with those in the empty plasmid group （0. 237 4 ± 0. 041 4） μg/mL, and normal saline control group （0. 199 3 ± 0. 089 9） μg/ mL. Therefore, orally take liposome-coated Ag85A DNA vaccine was able to induce the establishment of peripheral specific antibody and the increment of slgA level in local intestinal mucous membrane.

关 键 词：AG85A DNA疫苗 脂质体 

分 类 号：R392.11[医药卫生—免疫学]