豚鼠、家鸽和蟾蜍肝细胞内酸性磷酸酶定量组织化学分析  

A QUANTITATIVE HISTOCHEMICAL STUDY OF ACID PHOSPHATASE IN HEPATOCYTES OF GUINEA PIG, PIGEON AND TOAD

作　　者：周勉[1] 王启民[1] 郑世彬[1] 

机构地区：[1]第三军医大学组织学胚胎学教研室,重庆630038

出　　处：《解剖学报》1990年第1期82-86,共5页Acta Anatomica Sinica

摘　　要：本文采用Leitz MPV-3型显微分光光度计和Leitz TAS图像分析仪,对豚鼠、家鸽和蟾蜍肝小叶中央区和门管旁区肝细胞酸性磷酸酶的含量,进行了定量组织化学测定。结果表明,肝细胞酸性磷酸酶的含量以豚鼠最高,蟾蜍次之,家鸽最低。肝细胞酸性磷酸酶的平均分布密度,按豚鼠、家鸽和蟾蜍依次减小。本文对肝细胞酸性磷酸酶平均分布密度随着动物进化而增大的意义进行了讨论。Guinea pig, pigeon and toad are the vertebrates on different evolution level. The acid phosphatase content in the hepatocytes of centrilobular area and periportal area of hepatic lobules were quantitatively measured by microspectrophotometer and image analyser. The results showed that the content of acid phosphatase of hepatocytes was highest in guinea pig, lowest in pigeon, and toad in between the both. The average density of acid phosphatase distribution in the hepatocytes increases according to the evolution order. The significance of this phenomenon was discussed.

关 键 词：酸性磷酸酶 肝细胞 定量组织化学 

分 类 号：Q954.4[生物学—动物学]