小鼠胸腺上皮细胞系的细胞学鉴定  

CYTOLOGICAL IDENTIFICATION OF THE MURINE MTEC1 THYMUS EPITHELIAL CELL LINE

作　　者：吴江声[1] 张兰芬[1] 傅爱华[1] 陈慰峰[2] 

机构地区：[1]北京医科大学组织学胚胎学教研室,北京100083 [2]北京医科大学免疫学教研室,北京100083

出　　处：《解剖学报》1990年第4期408-410,共3页Acta Anatomica Sinica

摘　　要：本文报道小鼠胸腺上皮细胞系(MTEC_1系)的细胞学鉴定。细胞为多角形,呈上皮样排列,用抗角蛋白抗体显示细胞胞质内均含角蛋白。电镜观察可见胞质内含成束的张力细丝,相邻细胞间有桥粒结构。用抗小鼠上皮细胞单克隆抗体进行免疫组织化学染色,几乎全部MTEC_1细胞均为MTS33阳性,提示MTEC_1细胞是来源于胸腺體质的上皮样细胞。大多数MTEC_1细胞有正常小鼠染色体数(40)条。本文结果表明,MTEC_1细胞系属正常小鼠胸腺上皮细胞。A murine MTEC_1 thymus epithelial cell line established by us has been characterized. The cells were polyhydral and closely packed each other as epithelial like cells. Using anti-keratin antibody, the keratin were shown in cytoplasm of all cells. Under electronmicroscope, bunches of tonofilaments were clearly shown in the cytoplasm, and desmosomes were seen between neighbouring cells. Using anti-mouse epithelial cell monoclonal antibodies for immunohistochemical study, nearly all of the MTEC_1 cells were MTS33 positive. It suggests that MTEC, cells were derived from the epithelial cells located in medulla. The majority of the MTEC_1 cells have normal mouse diploid chomosome number of 40. These results provide evidence that MTEC, cell line is normal murine thymus epithelial cells.

关 键 词：胸腺 上皮细胞系 细胞学鉴定 

分 类 号：R322.53[医药卫生—人体解剖和组织胚胎学]