谷氨酰胺二肽对日本对虾血清生化指标、肝胰腺细胞凋亡及肠粘膜形态的影响  

BIOCHEMICAL EFFECTS OF GLUTAMINE DIPEPTIDE ON PENAEUS JAPONICUS

作　　者：叶均安[1] 王冰心[1] 孙红霞 李瑞丽[1] 伊祥华 周奕毅[1] 吴旧生[1] 刘波静[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江省象山县农林局,宁波315700 [3]宁波象山大目涂经济开发总公司,宁波315700

出　　处：《海洋与湖沼》2009年第3期347-352,共6页Oceanologia Et Limnologia Sinica

基　　金：国家科技部项目“对虾生物防病制剂及稳产养殖技术规模化应用示范”,04EFN213310124号;宁波市重大农业攻关专项,2007C10026号

摘　　要：采用饲养对比试验方法,研究了谷氨酰胺二肽(Gln二肽)对日本对虾血清生化指标、肝胰腺细胞凋亡和肠绒毛的影响及对虾体健康的作用。结果表明,饲喂Gln二肽各组的PO、LSZ、ACP含量均显著(P<0.05)高于对照组,添加0.5%和1.0%Gln二肽组的SOD和ALP含量也显著(P<0.05)高于对照组;添加1.0%Gln二肽的TP含量显著(P<0.05)高于其它各组。血清TP、PA、UN、CHO、PO、LSZ、SOD、ALP、ACP和LDH等各项指标均随Gln二肽添加量的提高而呈现增加趋势。试验建立了流式细胞术检测对虾肝胰腺细胞凋亡率的方法;肝胰腺细胞凋亡率随着日粮Gln二肽添加量的增加而显著降低(P<0.05)。肠道切片显微观测结果表明,添加1.0%的Gln二肽能显著增加日本对虾肠绒毛高度(P<0.05)。饲料中添加Gln二肽可显著提高日本对虾血清中的溶菌酶、抗氧化酶及磷酸酶活性,降低肝胰腺细胞凋亡率,增加肠绒毛高度,改善虾体健康状况。The effects of glutamine dipeptide on serum biochemical parameters, wither of pancreas cells, morphology of intestinal mucosa in Penaeus japonicus are studied. 240 individuals in 9--10cm in length and 11--14g in body weight were randomly assigned to four treatments in three replicates and 20 prawns in each tank. The groups received in oral administration the same basal diet supplemented of 0%, 0.25%, 0.5% or 1.0% glutamine dipeptide, respectively. The trial lasted for 16 days. The results show that adding glutamine-dipeptide significantly increased serum phenoloxidase, lysozyme, and acid phosphatase activity （P〈0.05）. The serum superoxide dismutase and alkatine phosphatase activity was significantly higher in with 0.5% or 1.0% groups than the control （P〈0.05）, and significantly higher total protein concentration in plasma of the 1.0% group than the control and others （P〈0.05）. In addition, increases were also found in plasma total protein, prealbumin, uric nitrogen, cholesterol, phenoloxidase activity, lysozyme activity, superoxide dismutase activity, alkatine phosphatase activity, acid phosphatase activity, and lactate dehydrogenase. The apoptosis of the hepatopancreas cells was established in flow cytometry, showing that the apoptosis was significantly reduced （P〈0.05）; adding 1.0% glutamine dipeptide significantly increased the intestinal villi height （P〈0.05）. Therefore, glutamine dipeptide could enhance the activity of lysozyme, antioxidant enzyme, and phosphatase in plasma, decrease the rate of apoptosis of hepatopancreatic cells, and increase intestinal villi height in P. japonicus; in other words, the healthy of prawns were improved.

关 键 词：谷氨酰胺二肽 日本对虾 总蛋白 抗氧化酶 细胞凋亡 肠粘膜形态 

分 类 号：S858.28[农业科学—临床兽医学] S831.5[农业科学—兽医学]