Cytogenetic and molecular identification of three Triticum aestivum-Leymus racemosus translocation addition lines  

作　　者：Le Wang Jianhua Yuan Tongde Bie Bo Zhou Peidu Chen 

机构地区：[1]National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China [2]Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China [3]Yangzhou Academy of Agricultural Sciences, Yangzhou 225007, China

出　　处：《Journal of Genetics and Genomics》2009年第6期379-385,共7页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30270827 and 30871519);the Science and Technology Project of Jiangsu Province (No. BG20053107);the 111 Project of the Ministry of Education of China;CCRP Program of the McKnight Foundation.

摘　　要：Chromosome 2C from Aegilops cylindrica has the ability to induce chromosome breakage in common wheat （Tritivum aestivum）. In the BC1F3 generation of the T. aestivum cv. Chinese Spring and a hybrid between T. aestivum-Leymus racemosus Lr.7 addition line and T. aestivum-Ae, cylindrica 2C addition line, three disomic translocation addition lines （2n = 44） were selected by mitotic chromosome C-banding and genomic in situ hybridization. We further characterized these T. aestivum-L, racemosus translocation addition lines, NAU636, NAU637 and NAU638, by chromosome C-banding, in situ hybridization using the A- and D-genome-specific bacterial artificial chromosome （BAC） clones 676D4 and 9M13; plasmids pAsl and pSc119.2, and 45S rDNA; as well as genomic DNA of L. racemosus as probes, in combination with double ditelosomic test cross and SSR marker analysis. The translocation chromosomes were designated as T3AS-Lr7S, T6BS-Lr7S, and T5DS-Lr7L. The translocation line T3AS-Lr7S was highly resistant to Fusarium head blight and will be useful germplasm for resistance breeding.Chromosome 2C from Aegilops cylindrica has the ability to induce chromosome breakage in common wheat （Tritivum aestivum）. In the BC1F3 generation of the T. aestivum cv. Chinese Spring and a hybrid between T. aestivum-Leymus racemosus Lr.7 addition line and T. aestivum-Ae, cylindrica 2C addition line, three disomic translocation addition lines （2n = 44） were selected by mitotic chromosome C-banding and genomic in situ hybridization. We further characterized these T. aestivum-L, racemosus translocation addition lines, NAU636, NAU637 and NAU638, by chromosome C-banding, in situ hybridization using the A- and D-genome-specific bacterial artificial chromosome （BAC） clones 676D4 and 9M13; plasmids pAsl and pSc119.2, and 45S rDNA; as well as genomic DNA of L. racemosus as probes, in combination with double ditelosomic test cross and SSR marker analysis. The translocation chromosomes were designated as T3AS-Lr7S, T6BS-Lr7S, and T5DS-Lr7L. The translocation line T3AS-Lr7S was highly resistant to Fusarium head blight and will be useful germplasm for resistance breeding.

关 键 词：WHEAT Leymus racemosus translocation line C-BANDING fluorescence in situ hybridization （FISH） test cross analysis 

分 类 号：S512.1[农业科学—作物学] Q78[生物学—分子生物学]