高效液相色谱法测定丙谷胺的有关物质  

Determination of Related Substances in Proglumide by HPLC

作　　者：多凯[1] 宋红波[1] 于新颖[1] 杨杨[1] 白政忠[1] 姜连阁[1] 

机构地区：[1]黑龙江省药品检验所,150001

出　　处：《黑龙江医药》2009年第3期245-246,共2页Heilongjiang Medicine journal

摘　　要：目的:探讨用高效液相色谱(HPLC)法测定丙谷胺的有关物质的量。方法:采用高效液相色谱法,色谱柱为Waters Symmetry C18(4.6mm×150mm,5μm);流动相为:2%乙酸铵-甲醇-乙腈(60:30:10);流速:lml/min;检测器:UV;检测波长:240nm。结果:在选定的色谱条件下,主峰和紧邻杂质峰能得到较好分离,精密度和理论塔板数很好。结论:HPLC法可用于丙谷胺有关物质的测定,方法简便、准确、稳定。Objective：To develop a HPLC method for the determination of the related substances in proglumide. Methods：A Waters Symmertry C18 column （4.6mm×150mm,5μm） was used with the mobile phase of 2% ammonium acetate-methanol-acetonitrile （60：30：10） at 1ml/min. The wavelength of UV detection was 240 nm. Results：Under this selected chromatograph condition, the prominent peak and the neighbor subsidiary peak could be successfully separated,and had very good accuracy and the theoretical plate number. Conclusion：This method is simple, accurate and stable. It can be applied to the determi- nation of the related substances in proglumide.

关 键 词：高效液相色谱法 丙谷胺 有关物质 

分 类 号：R927.2[医药卫生—药学]