苯扎贝特对肥胖和2型糖尿病大鼠胰岛β细胞保护的实验性研究  

The experimental study of protection to βcell of obese rats and type-2 diabetic rats by Bezafibrate treatment

作　　者：王艳[1] 赖晓阳[1] 张笠[1] 

机构地区：[1]南昌大学第二附属医院内分泌代谢科,南昌330006

出　　处：《江西医药》2009年第5期435-438,共4页Jiangxi Medical Journal

基　　金：2008年江西省卫生厅科技计划(编号:20081066)

摘　　要：目的观察苯扎贝特干预前后肥胖和2型糖尿病大鼠糖脂代谢、胰岛分泌功能的变化以及胰岛β细胞的凋亡情况。方法75只雄性SD大鼠随机分为空白对照组(NC,n=10),高脂饮食组(HF,n=65)喂养8周建立的肥胖模型再随机分为肥胖组和糖尿病组,2型糖尿病造模成功后,将上述两组再随机各分两组,分别为:单纯肥胖组(OB),肥胖+苯扎贝特(OB+BZ),糖尿病组(DM)和糖尿病+苯扎贝特组(DM+BZ),与NC组一同进入药物干预,测定药物干预前后FBG、TG、TC、FINs等指标,干预12周后处死大鼠,HE染色观察胰腺组织并采用TUNEL法计数凋亡胰岛β细胞。结果(1)肥胖组和糖尿病组大鼠合并高TG、TC血症,经苯扎贝特干预后血TG、TC明显下降(P<0.01),OB+BZ组FINs水平较干预前明显降低(P<0.01),DM+BZ组FBG获得改善,FINs水平得到恢复(P<0.05)。(2)OB、DM组均出现胰岛β细胞凋亡,DM组凋亡趋势更为显著,苯扎贝特干预后OB+BZ组较OB组、DM+BZ组较DM组均有明显改善(P<0.05)。结论肥胖和2型糖尿病大鼠多合并脂代谢紊乱并造成胰岛β细胞凋亡,苯扎贝特控制高脂血症可改善胰岛β细胞分泌功能和减少胰岛素抵抗,对胰岛β细胞脂性凋亡具有一定的保护作用,改善糖代谢。Objective To observe the changes of glycometabolism,lipid metabolism, secretary function of βcell and the apoptosis effect of βcell in obese rats and type-2 diabetic rats by the treatment of bezafibrate.nethods 75 Sprague-Dawley rats were divided into Normal control（NC,n=10） and High fat-fed diet（HF,n=65）.After the obese rats model had been formed,all rats were divided into two groups.One would be injected with STZ while the others wouldn＇t.The animal model of type 2 diabetes would be formed.Then they were divided into two groups again,there were normal contrel（NC）,obese rats（OB）,obese rats with bezafibrate（OB＋BZ）,diabetes rats （DM）and diabetes rats with bezafibrate（DM＋BZ）.To detect the levels of Fasting blood glucose（FBG）, Triglyceride（TG）, Total cholesterol （TC）, Fasting insulins （FINs） et al before and after the treatment of bezafibrate.After 12 weeks intervention,dyed with Hematoxylin- Eosin,the morphous of panerease islet were observed by microscope.The apoptotic βcells in islets were detected and quantified by the TUNEL technology.Results （1）The levels of TG,TC were decreased conspicuously in OB＋BZ and DM＋BZ groups.There shows lower levels of FINs in OB＋BZ group than pre-intervention[（31.81±2.18）mlu/l vs（22.33±2.85） mlu/l,P〈0.01],Levels of FBG in DM＋BZ group were deceded than pre-intervention [（19.93±1.85）mmol/l vs （10.57±1.73）mmol/l,P〈0.05],when the levels of FINs were improved [（15.84±1.73） mlu/l vs（19.50±1.64） mlu/l,P〈0.05]; （2）The rate of apoptotic βcells in islets were higher in OB and DM groups than in NC group which conspicuously higher in DM group.After the bezafibrate treatment,the rate of apoptotic βcells in islets were decreased both in OB＋BZ[（8.6±1.2） %vs（13.4±1.5）%,P〈0.05] and DM＋BZ[（12.6±2.1） %vs（22.6±2.5）%, P〈0.05] groups.Conclusion Long term high fat fed diet could induce the apoptosis of βcells in pancrease islet of rats,by intervening hyperlipemia with bez

关 键 词：Β细胞 苯扎贝特 脂毒性 肥胖 2型糖尿病 凋亡 

分 类 号：R587.1[医药卫生—内分泌]