新疆甘草斑点病病原分离鉴定  

Isolation and Identification of Pathogen of Liquorice Leaf-spot Disease in Xinjiang

作　　者：黄素芳[1,2] 向本春[1,2] 任毓忠[1,2] 李虎 

机构地区：[1]石河子大学农学院植物保护系,新疆石河子832003 [2]新疆兵团绿洲生态农业重点实验室,新疆石河子832003 [3]新疆维吾尔自治区植物保护站,乌鲁木齐830006

出　　处：《新疆农业科学》2009年第3期536-539,共4页Xinjiang Agricultural Sciences

基　　金：国家科技支撑计划项目(207BAC17B06)

摘　　要：2007年在甘草生长茂盛期,从石河子大学实验场的一实验地采集甘草斑点病病样,分别从甘草病样的茎和叶上分离纯化得到了7个菌株;使用喷雾接种健康甘草植株后,所有供试菌株均表现与田间自然发病相一致的症状;从中选取了1个代表性菌株进行了形态特征和培养特性的观察和测定,同时结合真菌18SrDNA通用引物进行了PCR扩增和序列测定,登录GenBank比对后,将其鉴定为草茎点霉(P.herbarumWestd),该病原菌在新疆甘草上的危害在新疆尚属首次报道。由于其危害性大,生产上应引起高度重视。Fungus were isolated from diseased Liquorice, collected in a field in Shihhotze of Xinjiang in 2007 and tested for the ability to cause comparable leaf- spot symptoms in Liquorice growth in a greenhouse. Some disease symptoms were reproduced with isolated fungus suspension when Liquorice were sprayed for two weeks. Pathogenic isolates were identified as P. herbarum Westd. based on morphological characterizations, physiological and 18S ribosomal DNA sequence analysis （98% nucleotide identity）. This is the first report that P. herbarum Westd. is the pathogen of Liquorice leaf- spot in Xinjiang. Because of the serious damage to Liquorice , greater attention should be payed to in production.

关 键 词：甘草 斑点病 病原鉴定 

分 类 号：S567[农业科学—中草药栽培]