雪花莲凝集素基因转化新疆甜瓜抗蚜虫的研究  

A Study on Transferring Snowdrop Galanthus nivalis(GNA) Gene in Cucumis melo L.of Xinjiang

作　　者：钟俐[1] 李群[1] 李冠[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《新疆农业科学》2009年第3期556-560,共5页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30660078);新疆维吾尔自治区高技术研究发展计划项目(200711102)

摘　　要：采用农杆菌介导法对新疆甜瓜进行了遗传转化研究,对影响转化频率的不同因素,即:抗生素浓度、预培养有无及感染时菌液的不同处理、外植体与农杆菌的感染时间和共培养时间等进行了研究,通过农杆菌介导法将雪花莲凝集素基因导入四日龄甜瓜子叶外植体,获得了卡那霉素的抗性植株。移栽成活并生长良好。卡那霉素抗性鉴定及转化株总DNA的PCR分析表明,GNA基因已整合到甜瓜的基因组中。初步抗蚜虫实验证明,转基因甜瓜有一定的抗棉蚜幼虫效果。This study was designed to investigate the genetic transformation of Cucumis melo L. by Agrobacterium- mediated trans formation. Some factors affecting the Agrobacterium - mediated transformation frequency, i. e. concentrations of antibiotics, preculture of cotyledon explants, treatments of Agrobacterium and infection time, co-infection time were also discussed. GNA Gene was translated into melon by Agrobacterium - mediated transformation , The kanamycin-resistant plants have been regenerated. They survived after transplantation and grew well in pots. Transgenic plants were confirmed by the kanamycin- resistant experimen and PCR analysis. The preliminary results from bioassay demonstrated significant resistance of the transgenic plants to cotton aphide larvae.

关 键 词：甜瓜 雪花莲凝集素基因 农杆菌介导法 

分 类 号：S152[农业科学—土壤学]