利用60%季铵化聚(4-乙烯)吡啶固定化酶的电位型尿素酶电极的研制  

Preparation of Potentiometric Enzyme Electrode for Urea Based on Enzyme Immobilization by 60% Quaternized Poly(4 vinyl)pyridine

作　　者：李文江[1] 刘潇雅 孙长青[1] 

机构地区：[1]吉林大学化学系

出　　处：《高等学校化学学报》1998年第5期711-713,共3页Chemical Journal of Chinese Universities

基　　金：吉林省科学技术委员会科学基金

摘　　要：在电位型尿素酶电极的组装过程中，要求尽量不改变尿素酶的构型和构象，使其在酶膜中保持其自然状态，从而可获得较高的酶活力．用ｐＨ玻璃电极作原电极，将尿素酶固定在其表面，常用的有戊二醛交联法［１］和各种聚合物膜法［２～４］．本文利用６０％季铵化的聚（４－乙...Based on electrostatic interaction between 60% quaternized poly(4 vinyl)pyridine and urease molecules, which can be immobilized on the surface of pH glassy electrode. By means of this technique, the enzyme electrode for urea was prepared. In experiments, we discussed in detail effects of kinds, concentration and pH of media on response of the electrode. In 0.025 mol/L phosphate buffer solution(pH 6.8),the linearity range is between 1×10 -4 and 6×10 -2 mol/L and the detection limit is 1×10 -4 mol/L. The electrode has been applied to the determination of urea in standard serum and recovery is between 92.9% and 107 0%.

关 键 词：电位法 尿素酶电极 尿素测定 分析 

分 类 号：O623.663[理学—有机化学] R446.111[理学—化学]