RP-HPLC内标法测定土壤中甲磺隆和氯磺隆的残留量  

Determination of Metsulfuronmethyl and Chlorosulfuron in Soil by RPHPLC with Internal Standard Method

作　　者：张秀真[1] 符素敏 高素莲[1] 方娟娟[1] 

机构地区：[1]安徽大学现代实验技术中心国家计量认证实验室,安徽合肥230039 [2]安徽安凯福田曙光车桥有限公司,安徽合肥230051

出　　处：《化学研究》2009年第2期98-100,共3页Chemical Research

基　　金：安徽省重点科研资助项目(05021026)

摘　　要：采用反相高效液相色谱-内标法测定了土壤中甲磺隆和氯磺隆的含量．以苯甲醇为内标物，C18反相柱（250mm×4．6mm i．d．，5μm）为分析柱，甲醇／1％醋酸钠水溶液（Ф=50／50）为流动相，流速1．0mL/min；检测波长254nm，柱温30℃．在甲磺隆浓度3．92-39．2mg／L，氯磺隆浓度2．02～20．2mg／L范围内，各对照品与苯甲醇的色谱峰面积比呈良好的线性关系．该方法准确、快速、灵敏度高、重现性好．The determination method of the content of metsulfuron-methyl and chlorosulfuron in soil was established utilizing reverse phase high performance liquid chromatography （RP-HPLC） by taking benzyl alcohol as an internal standard. The RP-HPLC was conducted on C18 reverse phase column （250 mm × 4.6 mm i. d., 5 μm） with methanol / 1% NaAc solution （ Ф = 50/50） as mobile phase. The flow rate of mobile phase was 1.0 mL/min. The detective wavelength was set to 254 nm and column temperature at 30 ℃. The chromatography peak area ratio of standard to the internal standard was very well correlated with the concentration of metsulfuron-methyl in the range of 3.92- 39.2 mg/L and chlorsulfuron in the range of 2.02 - 20.2 mg/L. The method is simple, accurate and sensitive and can be applied to the determination of metsulfuron-methyl and chlorsulfuron in soil with satisfactory results.

关 键 词：甲磺隆 氯磺隆 除草剂 HPLC 内标法 

分 类 号：S482.4[农业科学—农药学] O657.7[农业科学—植物保护]