高速逆流色谱法分离制备华山参中东莨菪内酯  

Isolation of Scopoletin from Physochlaine Infundibularis Kuang by High-speed Counter-current Chromatography

作　　者：刘彩霞[1] 呼亚旭[1] 李宁宁[1] 赵东保[1,2] 王勇[1] 刘绣华[1] 

机构地区：[1]河南大学化学化工学院河南省天然药物与免疫工程重点实验室,河南开封475004 [2]济源职业技术学院,河南济源454650

出　　处：《化学研究》2009年第2期101-103,共3页Chemical Research

基　　金：河南省教育厅自然科学基金项目(2007150030);河南省科技厅国际合作项目(084300510023)

摘　　要：采用高速逆流色谱法从华山参乙醇提取液的氯仿萃取物中分离得到了一种高纯度的香豆素类化合物,东莨菪内酯.所用体系为正己烷∶乙酸乙酯∶甲醇∶水(体积比3∶5∶3∶5),上相做固定相,下相做流动相.分离所得东莨菪内酯的纯度经高效液相色谱检测达到97%.该方法操作简单、方便、快捷,可用于华山参中香豆素类化合物——东莨菪内酯的分离制备.A high-speed counter-current chromatography method for isolation of coumarin from physochlaine infundibularis Kuang was developed by using hexane-ethyl acetate-methanol-water as the twophase solvent system in elution at an optimized volume ratio of 3：5： 3：5. The upper phase of the solvent system was used as stationary phase, and the lower phase was used as mobile phase. The purity of scopoletin from the separated fraction was 97% analyzed by HPLC. The method is easy to operate with low cost and can be used for separation and preparation of scopoletin from physochlaine infundibularis Kuang.

关 键 词：华山参 香豆素 东莨菪内酯 高速逆流色谱 高效液相色谱 

分 类 号：O623.7[理学—有机化学]