梅花鹿粪便中苏云金杆菌分离株的生物学特性及其cry基因型鉴定  

Biological Characteristics of Bacillus Thuringiensis Isolated from the Feces of a Sika Deer and Identification of Its Cry-type Genes

作　　者：吴昌标[1,2] 关怡[1] 邱津津[1] 黄张敏[1] 张爱华[3] 

机构地区：[1]福建农林大学教育部生物农药与化学生物学重点实验室,福州350002 [2]福建农业职业技术学院,福州350119 [3]福建农业科学院畜牧兽医研究所,福州350013

出　　处：《经济动物学报》2009年第2期72-76,共5页Journal of Economic Animal

基　　金：国家863课题(2006AA10A212);福建省科技厅重点项目(2008N0050);福建农业职业技术学院重点课题(08-EM-01);福建省大学生科技创新项目

摘　　要：从福州动物园采集梅花鹿粪便,采用醋酸钠-抗生素分离法分离到1株苏云金杆菌(Bacillus thuringiensis,简称Bt)MHL0702,为了解该新菌株的生物学特性,对其进行了形态学、生理生化指标研究;通过PCR-RFLP鉴定体系对其cry基因型进行分析。结果表明:该菌株含有cry1Aa、cry1Ba、cry1Ea和cry1Ia类基因。对3日龄小菜蛾幼虫的室内毒力测定结果显示,该菌株对重要的农业害虫小菜蛾有较高的杀虫毒力。The strain Bt MHL0702 was isolated using NaAc and antibiotics from the feces of a Sika deer in Fuzhou Zoo, Fujian, China. The morphology and biochemical reactions of this strain were conducted, and its cry-type genes had been identified using PCR-RFLP system. The evidence showed that this isolate harbored crylAa, crylBa, crylEa and crylIa genes. The toxicity determination in laboratory showed MHL0702 was high toxicity against the agricultural pest Plutella xylostella.

关 键 词：梅花鹿 苏云金杆菌 分离 CRY基因 

分 类 号：Q93-331[生物学—微生物学]