包合锰卟啉的环糊精聚合物作为过氧化物朊酶模型物的研究  

Inclusive Complex of Manganese-porphyrin with β-Cyclodextrin Polymer as an Analogue for Peroxide Proteinase

作　　者：李荣[1] 毛陆原[1] 朱敏[1] 黄雪梅[1] 沈含熙[1] 

机构地区：[1]南开大学化学系

出　　处：《分析科学学报》1998年第2期94-97,共4页Journal of Analytical Science

基　　金：国家自然科学基金

摘　　要：制备了β－环糊精交联聚合物（β－ＣＤＰ）和四（４－三甲铵基苯基）卟啉锰（ＭｎＴＡＰＰ）的超分子包合物（ＭｎＴＡＰＰ－β－ＣＤＰ），并以此包合物作为过氧化物朊酶的模型物．结果表明，固载化的包合物不仅十分稳定，而且呈现出较高的过氧化物朊酶活性．将它应用于酶法分析，可催化过氧化氢与４－氨基安替比林和对氯苯酚的显色反应，当过氧化氢浓度为０～２．５μｇ／ｍＬ时，与吸光度呈良好线性关系，相关系数为０．９９９２．The supramolecular inclusive complex of 5,10,15,20-tetrakis[4-(trimethylammonium)pheny1]-21H,23H-porphine manganese (Ⅱ)(MnTAPP)with β-cyclodextrin cross-linking polymer (β-CDP) was obtained and used as an analogue for peroxide proteinase. The immobilized inclusive complex as a mimetic enzyme of horseradish peroxidase was very stable exhibited the high proteinase-like activity, which has been demonstrated by enzymatic methods of analysis. The method was applied to the determination of hydrogen peroxide with 4-aminoantipyrine and p-chlorophenic acid. The calibration graph for the determination of hydrogen peroxide was linear from 0 to 2.5μg/mL and the linear correlation coefficient was found as 0.9992.

关 键 词：过氧化氢 测定 分析 模型物 模拟 过氧化物朊酶 

分 类 号：O613.3[理学—无机化学] Q554.6[理学—化学]