大孔吸附树脂纯化迷迭香脂溶性总酚酸的研究  

STUDY ON PURIFICATION OF LIPOSOLUBLE TOTAL PHENOLIC ACIDS FROM ROSEMARY BY MACROPOROUS RESIN

作　　者：杨磊[1] 隋小宇[1] 马春慧[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《离子交换与吸附》2009年第3期225-232,共8页Ion Exchange and Adsorption

基　　金："十一五"国家科技支撑计划项目(2006BAD18B04)

摘　　要：从X-5、D4020、AB-8、H1020、NKA-II、HPD-100A、SIPI、HPD800和D3520大孔吸附树脂中筛选出H1020树脂,研究了其对迷迭香脂溶性总酚酸的静态与动态吸附和解吸性能。结果表明,H1020树脂对迷迭香脂溶性总酚酸的饱和吸附量为19.84mg/g干树脂,饱和吸附时间为3h,适宜的解吸剂为体积分数90%的乙醇溶液;以质量浓度为4.45mg/mL的迷迭香提取液上柱,流速为1.0mL/min,当吸附平衡后,2.7BV体积分数90%的乙醇溶液可将吸附的总酚酸完全洗脱。经动态纯化后,脂溶性总酚酸质量分数从47.74%提高到70.46%,该组分清除DPPH自由基的IC50值为0.0469mg/mL。H1020 resin was screened out from 9 kinds of macroporous resin including X-5, D4020, AB-8, H1020, NKA-II, I-IPD-100A, SIPI, I-IPD800 and D3520; it was the optimum maeroporous resin to purify liposoluble total phenolic acids from rosemary. The results showed saturated adsorption capacity of H1020 resin was 19.84mg/g （dry resin）, saturated adsorption time was 3h, suitable desorption agent was 90% （volume fraction） ethanol solution; the flow rate was lmL/min when the concentration of rosemary total phenolic acids was 4.45mg/mL, after reaction reached adsorption equilibrium, adsorbed total phenolic acids was completely eluted by 90% ethanol solution with 2.7BV. After dynamic purification, the liposoluble total phenolic acids mass fraction increased from 47.74% to 70.46%, and its IC50 value on scavenging DPPH radical was 0.0469mg/mL.

关 键 词：迷迭香 总酚酸 大孔吸附树脂 DPPH 

分 类 号：TQ028[化学工程]