新型大孔硅质酰胺型聚合物键合相的制备及其对蛋白质的分离  

Preparation of Novel Macroporous Silica Based Amide Polymer Bonded Packing and Its Application to the Separation of Proteins

作　　者：魏芸[1] 樊立民[1] 蒋生祥[1] 陈立仁[1] 

机构地区：[1]中国科学院兰州化学物理研究所

出　　处：《色谱》1998年第3期191-194,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金

摘　　要：大孔硅胶与乙烯基硅烷反应后，再与甲基丙烯酰胺和二乙烯基苯共聚成一种新型分离蛋白质的色谱柱填料。考察了这种色谱填料对蛋白质的分离能力，认为其具有柱效高、惰性好和分离效率高的优点，聚合物键合相的制备重复性好。A novel macroporous silica based amide polymer bonded packing for protein separations in HPLC is described.The macroporous silica support was bonded with diethoxymethyl vinyl silane and then copolymerized with methylacrylamide and divinylbenzene to produce a tailored stationary phase with high resolution and inertness.The repeatability of packing preparation is good.They were characterized through the application of a standard protein mixture of pepsin,glucose oxidase,bean trypsinogen inhibitor and ribonuclease.The time for elution of these proteins is less than 12 minutes.It is suggested that the macroporous silica based amide polymer bonded packing can be used to quickly separate biopolymer.This packing is a better alternative for the biopolymer separation.

关 键 词：固定相 分析 蛋白质 分离 色谱柱填料 填料 

分 类 号：Q510.3[生物学—生物化学] O657.703[理学—分析化学]