瓜蒌纤溶酶的分离纯化及性质研究  

Study on The Purification and Properties of Fibrinolytic Enzyme in Trichosanthes kirilowii

作　　者：李尧[1] 周艳芬[1,2] 郭晓军[3] 胖铁良[3] 朱宝成[1,3] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北省生物工程研究中心,河北保定071002 [3]河北农业大学生命科学学院,河北保定071001

出　　处：《畜牧与饲料科学》2009年第4期23-25,共3页Animal Husbandry and Feed Science

摘　　要：利用亲和层析从瓜蒌蛋白粗提液中分离纯化得到一纤溶酶,并对其部分酶学性质进行研究。结果表明,该酶表观分子量为66.0kD;最适pH值为9.0;在4～40℃活性无明显变化,78℃以上完全失活;在以酪蛋白为底物的水解反应中,温度、酶底物比、pH值、反应时间4种因素对水解反应的影响力大小为:温度>酶底物比>反应时间>pH;Km值为1.89mg/mL,Vmax=555.56μmol/(L·min)。The fibrinolytic enzyme was separated and purified from protein crude extracts, of Trichosanthes kirilowii by using affinity chromatography method and its enzymatic properties were studied. The results showed that the apparent molecular weight of the fibrinolytic enzyme was 66.0 kD, the optimal pH value was 9.0, and the enzyme activities had no significant changes under the temperature of 4-40℃ and was inactivate completely when temperature was more than 78℃. In the hydrolysis reaction with the casein as substrate, the effects of 4 kinds of factors such as temperature, enzyme substrate ratio, pH value and reaction time on the hydrolysis reaction were in order as follows： temperature〉enzyme substrate ratio〉reaction time 〉 pH value; the Km value was 1.89 mg/mL and Vmax was 555.56μmol/（ L·min）.

关 键 词：瓜蒌 纤溶酶 亲和层析 KM值 

分 类 号：Q556[生物学—生物化学]