普鲁士蓝和苝四甲酸二酐衍生物膜修饰的过氧化氢生物传感器  被引量:4

Hydrogen Peroxide Biosensor Modified by Prussian Blue and Derivative of 3,4,9,10-Perylenetetracarboxylic Dianhydride

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作  者:李华刚[1,2] 袁若[1] 柴雅琴[1] 李文娟[1] 苗向敏[1] 卓颖[1] 洪成林[1] 

机构地区:[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715 [2]遵义师范学院化学系,遵义563002

出  处:《分析化学》2009年第6期806-810,共5页Chinese Journal of Analytical Chemistry

基  金:国家自然科学基金(No.20675064);重庆市自然科学基金(No.CSTC-2005BB4100)资助项目

摘  要:以玻碳电极(GCE)为基底,采用恒电位法沉积一层普鲁士蓝(PB),然后将苝四甲酸二酐衍生物(PTC-NH2)自组装到其表面,形成既带氨基功能团,又可有效防止PB渗漏的导电膜。通过静电吸附和共价键合作用固定纳米金和辣根过氧化物酶(HRP)的复合物,从而制得性能优良的过氧化氢(H2O2)生物传感器。采用循环伏安法(CV)和计时电流法,考察了传感器的电化学性能。实验表明,本传感器具有灵敏度高、线性范围宽、检出限低、稳定性好、抗干扰能力强等特点。其线性范围为2.0×10-6~1.4×10-3mol/L;检出限为8.3×10-7mol/L(S/N=3)。A novel type of hydrogen peroxide biosensor was prepared. First, a stable Prussian Blue (PB) film was electrodeposited on glassy carbon electrode by applying constant potential. After that, the derivative of 3,4,9,10-perylenetetracarboxylic dianhydride containing amino group was immobilized on the surface of PB by self-assembly, which can effectively prevent the leakage of PB. Then the composite of nano-gold and horseradish peroxidase (HRP-Au) was successful immobilized onto the above prepared film to construct the hydrogen peroxide biosensor via the effect of electrostatic adsorption and process of covalent bonding. The per- formance of the biosensor was characterized by cyclic voltammetry and the chronoamperometry. The proposed biosensor exhibited high sensitivity, broader linear range, a lower detection, excellent stability as well as good selectivity for H202 determination. And the linear range was from 2.0×10^-6~1.4×10^-5mol/L with a detection limit of 8.3 ×10^-7 mol/L( S/N = 3).

关 键 词:普鲁士蓝 苝四甲酸二酐衍生物 纳米金 辣根过氧化物酶 生物传感器 化学修饰电极 

分 类 号:TP212.2[自动化与计算机技术—检测技术与自动化装置]

 

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