乙型肝炎血清学标志物与HBV-DNA含量关系的探讨  

Study on the Relationship between HBV Serum Markers and the HBV-DNA Content

作　　者：黄超群[1] 杨小兰[1] 黄春花[1] 

机构地区：[1]广东省惠州市人民医院检验科,516002

出　　处：《医学理论与实践》2009年第6期629-632,共4页The Journal of Medical Theory and Practice

摘　　要：目的：为了进一步探讨乙型肝炎患者血清中HBV-DNA含量与HBV血清学标志物之间的关系，了解荧光定量聚合酶链反应（FQ-PCR）检测乙型肝炎病毒的应用价值。方法：应用酶联免疫吸附试验（ELISA法）检测510例来自我院门诊就诊者和住院患者血清标本中的特异性抗原抗体，并同时应用荧光定量PCR分析系统测定血清标本中HBV-DNA的含量进行分析。结果：经ELISA法与FQPCR法检测的510例血清标本中，51例“大三阳”（HBsAg＋HBeAg＋HBcAb）的血清标本，其HBV—DNA检出数均呈阳性，阳性率为100％，其HBV-DNA的拷贝数范围为10^5～10^9／ml 76例“小三阳”（HBsAg＋HBeAb＋HBcAb）的血清标本，其HBV-DNA检出的阳性率为47．4％，HBV-DNA的拷贝数范围为10^4～10^7／ml。结论：HBV-DNA是乙型肝炎病毒感染和复制的特异性指标。应用FQ-PCR技术检测乙型肝炎病毒，具有较高的灵敏度和特异性，其操作简便，定量准确，结果可靠，它比HBV血清学标志物更能反映乙型肝炎病毒在体内的存在和真实复制状况，在HBV-DNA感染诊断，特别是药物疗效的观察中，具有良好的应用价值。因此，HBV-DNA与血清学标志物的相互关系是本文研究的课题。Objective： To further study on the relationship between HBV serum markers and the HBV-DNA content in the serum of hepatitis B patients,and investigate the value of fluorescence quantitative polymerase chain reaction （FQ PCR） for detection of hepatitis B virus. Methods： Specific antigen and antibody in serum samples from 510 outpatients and inpatients was detected by enzyme-linked irnmunosorbent assay （ELISA）, and the HBV-DNA content in samples was detected and analyzed by fluorescence quantitative polymerase chain reaction（FQ-PCR）. Results.. In 510 serum sampies detected by ELISA and FQ-PCR,the detected numbers of HBV-DNA in 51 serum samples, the results of which were HBsAg＋HBeAg＋HBcAb, exceeded the normal value, and the range of copy number of HBV-DNA in samples was 10^5 - 10^9/ml. And the range of copy numbers of HBV-DNA in 76 serum samples, the results of which were HBsAg＋HBeAb＋HBcAb, was 10^4- 10^7/ml. The positive rate of the former was 100%, and that of the latter was 47.4%. Conclusion： HBV-DNA is the specific index Of the viral infection and copy. Fluorescence quantitative polymer- ase chain reaction （FQ-PCR） for detection of HBV is easily operated, highly sensitive and specific. Its results are reliable, and the HBV-DNA content detected by the reaction is accurate. The results of fluorescence quantitative polymerase chain reaction could better reflect the status of viral replication and existence than the level of HBV serum mgrkers in vivo, and the method, which is fluorescence quantitative polymerase chain reaction （FQ-PCR） for detection of HBV, is much valuable in HBV-DNA infection diagnosis, especially in the observation of drug effect. Therefore, the relationship between HBV-DNA and serum markers is the task discussed.

关 键 词：乙型肝炎病毒血清学标志物聚合酶链反应HBV-DNA定量 

分 类 号：R512.62[医药卫生—内科学]