毛细管电泳安培测定鼠单个交感神经细胞中儿茶酚胺类神经递质  

Determination of Catecholamines in Single Sympathetic Nerve Cell of Rat by Capillary Electrophoresis With Amperometric Detection

作　　者：胡深[1] 庞代文[1] 王宗礼[1] 程介克[1] 李之望[2] 樊友珍[2] 胡宏镇[2] 

机构地区：[1]武汉大学化学学院分析科学研究中心,武汉430072 [2]同济医科大学实验医学研究中心,武汉430030

出　　处：《分析化学》1998年第6期752-756,共5页Chinese Journal of Analytical Chemistry

摘　　要：用自组装的毛细管电泳微电极安培检测系统，分析了鼠的单个交感神经细胞。大白鼠经麻醉、微解剖及酶解分离出新鲜的单个交感神经细胞。采用全细胞进样，用异丙肾上腺素 作内标。测定了儿茶酚胺类神经递质去甲肾上腺素和肾上腺素。检测限分别达 ５７ ａｍｏｌ和 ５９ ａｍｏｌ。八个活细胞分析结果，每个细胞平均含去甲肾上腺素 ４５５ ａｍｏｌ及肾上腺素 ６５ ａｍｏｌ。This paper reports the use of a home-made capillary electrophoresis (CE) with amperometric detection system to the analysis of neurotransmitters in a single sympathetic nerve cell (diameter 15-25 um, volume 2-5 pL) of rat. After enzymolysis the cells were isolated. Sampling of the fresh whole cell was performed through electromigration by directly drawing a cell into the tip of a capillary and lysing the cell inside the capilary as observed under the microscope. The catecholamines in a single cell were separated by CE at the sepa- ration potential of 15 kV. Electrochemical detection was performed at 0. 7 V vs. SCE. Isoproterenol was selected to be the internal standard for the determination of norepinephrine (NE) and epinephrine (E) in a single sympathetic nerve cell. The detection limits for NE and E are 57 amol and 59 amol, respectively. The average analytical results of NE and E in eight single sympathetic nerve cells of rats are 445 amol and 65 amol/per cell, respectively.

关 键 词：分析 儿茶酚胺 神经递质 去甲肾上腺素 肾上腺素 

分 类 号：Q422[生物学—神经生物学]