卵泡液中总蛋白含量与卵泡直径的关系  

Relationship between Total Protein Content in Follicular Fluids and Follicle Diameter

作　　者：孙艳玲[1] 李纯锦[1] 陈璐[1] 李晓艳[2] 周虚[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国畜牧杂志》2009年第11期15-17,共3页Chinese Journal of Animal Science

基　　金：国家科技支撑项目(2006BAD14B08-01)

摘　　要：通过检测不同直径卵泡的卵泡液中总蛋白含量的变化，从而进一步阐明卵泡液中蛋白成分在卵泡发育过程中的作用。将直径为3-5、5-8、8—10mm的卵泡和囊肿卵泡（〉21mm）分为4组（n=10），分别抽取卵泡液。通过TCA／丙酮方法进行样品处理；根据Bradford法检测原理，利用分光光度计对其进行蛋白定量研究。结果表明：3-5mm为4440μg／mL，5-8mm／11为976μg／mL，8～10mm为686μg／mL，囊肿卵泡（〉21mm）为537．2μg／mL。经SPSS13．0软件分析，3—5mm卵泡液中蛋白平均浓度高于其他组（p〈0．01）；其他3组间差异均不显著（P〉0．05）；囊肿卵泡中蛋白含量最低。由此可得出，卵泡液中总蛋白浓度随着卵泡直径的增加而降低，表明卵泡液中的蛋白含量与卵泡发育的成熟度呈负相关。而囊肿卵泡的发生很可能和蛋白含量降低有关，为卵泡囊肿发病机制的研究提供新的理论参考。In order to clarify the function of protein in follicular fluids from sows during follicular development, we detected the changes of total protein content in different diameter follicles. The experiments were separated into four groups of small follicles（3-5 mm diameter）, middle follicles（5-8 mm diameter）, large follicles（8-10 mm diameter）, and cystic follicles（diameter 〉21mm）. Follicular fluids were collected from different diameter of the follicles with syringe. The protein samples in follicular fluids were extracted by the method of TCA/acetone pecipitation and quantificated by ProtaQuant Assay Kit. The mean concentrations of protein for the fluids were 4 440μg/mL for small follicles （3-5 mm diameter）, 976 μg/mL for middle follicles （5-8 mm diameter）, 686 μg/mL for large follicles （8-10 mm diameter）, and 537.2μg/mL for cystic follicles （diameter 〉21mm）. The results indicated that total protein concentrations in follicular fluids of 3-5 mm were significantly higher than those of other groups （P 〈 0.01 ） ;there were no differences among other groups （P 〉 0.05）;the concentrations of cystic fluids were lowest. Variability in the concentrations of protein within follilces suggested that total protein content was decreased as follicle diameter increased. The change of protein concentrations may be related to the follicle maturation. Lower protein concentration in cystic fluids would give new theoretical foundation to the pathology and etiology of ovarian follicular cysts.

关 键 词：卵泡液 蛋白 卵泡直径 关系 

分 类 号：S831.3[农业科学—畜牧学]