花鲈皮肤溃疡病抗性和敏感群体的RAPD分析  

Genetic diversity analysis based on RAPD for resistance and susceptibility stocks of Lateolabrax japonicus to the skin ulcer disease

作　　者：薛良义[1] 杨巧一[1] 韦家永[1] 李卢[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《海洋科学》2009年第6期61-66,共6页Marine Sciences

基　　金：浙江省自然科学基金资助项目(302438);宁波市青年基金资助项目(2003A62023)

摘　　要：采用随机扩增多态性DNA技术,对花鲈(Lateolabrax japonicus)皮肤溃疡病抗性和敏感两个群体的遗传多样性进行了研究。从100个10 bp随机引物中筛选出25个随机引物,在两个群体中共扩增了326个位点,抗性群体多态位点比率为44.24%,遗传变异度为0.123 7,Shannon多样性值为0.1149,分别比敏感群体高3.87%,5.82%和2.68%,表明花鲈抗病群体比敏感群体具有更丰富的遗传多样性。25条随机引物中,7条引物在两群体中的扩增结果存在差异,其中S11,S15,S4,S414,S416,S9在抗性群体的扩增结果比敏感群体多1个多态位点,S52多2个多态位点。在这8个特异多态位点中,S52-1,S4-1,S414-6,S9-2在敏感群体中全部出现,呈单态性;S15-5,S11-6,S416-6,S52-5只在抗性群体中出现,出现频率分别为38.5%,30.8%,23.1%和23.1%。说明这8个位点在两群体中存在较大的差异。研究表明,花鲈皮肤溃疡病在遗传上是比较复杂的,并不是简单地与某个遗传标记有关,可能与多个遗传因素相关。The genetic diversity of resistance and susceptibility stocks of Lateolabrax japonicus to the skin ulcer disease was studied by using randomly amplified polymorphic DNA （RAPD） method. Twenty-five random primers, which were screened from one hundred ones, were used for the analysis of nuclear DNA polymorphism of cultured L. japonicus in Xiangshan Bay. A total of 326 RAPD bands （loci） were detected in the two stocks. In the resistance stock, the proportion of polymorphic loci was 44.24%. The mean genetic variation index and Shannon Weaveis value of phenotypic diversity were 0. 123 7 and 0. 114 9, respectively. They were 3.87%, 5.82% and 2.68% higher than that of the suscepbility stock respectively. It was suggested that the genetic diversity of resistance stock was more abundant than that of suscepbility population. Seven of twenty-five primers generated different amplification patterns between the two stocks. In the resistance stock, six primers, Sll, S15, S4, S414, S416 and S9, amplify 1 polymorphic locus more than the suscepbility stock respectively, and primer S52 amplify 2 polymorphic loci more than the suscepbility stock. In the above 8 polymorphic loci, S15-5, S11-6, S416-6 and S52-5 bands only existed in the resistance stock, which suggested genetic differences between two stocks. However, the relationship between these oolymorphic loci and the disease resistance needs to be further investigated.

关 键 词：花鲈(Lateolabrax japonicus) 群体 皮肤溃疡病 RAPD 遗传多样性 

分 类 号：Q953[生物学—动物学]