茵栀黄注射液指纹图谱的研究  

Study on HPLC Fingerprint of Yinzhihuang Injection

作　　者：王柯[1] 张彤[2] 刘云[3] 毛秀红[1] 水彩虹[3] 徐莲英[2] 季申[1] 

机构地区：[1]上海市食品药品检验所,上海201203 [2]上海中医药大学,上海201203 [3]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《中国药学杂志》2009年第10期743-746,共4页Chinese Pharmaceutical Journal

基　　金：国家十五重大科技专项课题子课题资助项目(2001BA701A56)

摘　　要：目的建立评价茵栀黄注射液质量的指纹图谱分析方法。方法高效液相色谱条件Kromasil 100-5C_(18)色谱柱(4.6 mm×25 cm,5μm);甲醇-0.05%磷酸溶液为流动相进行二元梯度洗脱;流速1.0 mL·min^(-1);检测波长为238nm;柱温30℃。结果建立了茵栀黄注射液中绿原酸类、栀子苷类、黄芩苷类成分的指纹图谱,并建立了共有峰模式、全谱相似度和去黄芩苷相似度模式3种指纹图谱分析评价方法。结论建立的茵栀黄注射液指纹图谱的重现性、稳定性好,可以有效地控制茵栀黄注射液的质量。OBJECTIVE To establish a method for determining the fingerprints of Yinzhihuang Injection by HPLC. METHODS The separation was performed on a Kromasil 100-5C18 column （4.6 mm × 25 cm, 5 μm） with linear gradient elution. The flow rate was 1.0mL · min^-1 and the mobile phase consisted of methanol and 0.05% phosphoric acid solution. The detection wavelength was at 238nm and the temperature was at 30 ℃. RESULTS Three kinds of components in Yinzhihuang Injection were identified as chlorogenic acid, geniposide and Baicalin by fingerprints. Three evaluate methods for fingerprints of Yinzhihuang Injection were established, including the common peaks model, similarity degree model of whole fingerprints and similarity degree model deducted the peak ofbaicalin. CONCLUSION The HPLC fingerprints of Yinzhihuang Injection is stable and reproducible, and it can control the quality of Yinzhihuang Injection effectively.

关 键 词：高效液相色谱法 指纹图谱 茵栀黄注射液 

分 类 号：R284[医药卫生—中药学]